Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, PO Box 80260, 21589, Saudi Arabia; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, PO. Box 61511, 61519, Egypt.
Department of Marine Chemistry, Faculty of Marine Science, King Abdulaziz University, Jeddah, PO. Box 80207, 21589, Saudi Arabia.
J Ethnopharmacol. 2020 Jan 30;247:112278. doi: 10.1016/j.jep.2019.112278. Epub 2019 Oct 4.
Euryops arabicus (Asteraceae) is grown in Arab Peninsula. Its aerial parts possess ethnomedicinal applications against several inflammatory conditions.
To evaluate the anti-inflammatory activity of Euryops arabicus (E. arabicus) organic extract as well as its major polymethoxylated flavonoids.
Acute toxicity of the total extract of E. ararbicus was evaluated by assessing LD. In vivo anti-inflammatory activity was evaluated in rats injected with carrageenan in the plantar area. Paw edema volume, histological changes and rats'stair climbing and motility were assessed. In vitro anti-inflammatory activity of the isolated compounds was evaluated in peripheral blood mononuclear cells (PBMCs) challenged with carrageenan. Inflammation markers were assessed in cellular lysates and collected media.
The extract was found safe and considered unclassified with an oral LD > 2000 mg/kg in rats. Pretreatment of rats with a total extract of E. arabicus at doses of 100 and 250 mg/kg significantly inhibited carrageenan-induced increase in paw edema volume and histopathological changes. Also, it significantly ameliorated diminution of climbing and motility. Phytochemical studies led to the isolation and identification of five polymethoxylated flavonoids. The anti-inflammatory properties of the isolated compounds were evaluated in carrageenan-challenged peripheral blood mononuclear cells (PBMCs). All compounds exhibited appreciable antioxidant activities. Further, pre-incubation of the cells with the isolated metabolites significantly ameliorated the rise in cyclo-oxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and monocyte chemoattractant protein-1 (MCP-1) induced by carrageenan challenge. Further, the compounds inhibited the leakage of interleukin-1β (IL-1β), interleukin-6 (IL-6) and myeloperoxidase (MPO) in media collected from stimulated cells.
E. arabicus exhibited in vivo anti-inflammatory effects in the carrageenan model as it ameliorated rat paw edema, histopathological changes and movement dysfunction. in vitro activity of isolated compounds was confirmed in stimulated PBMCs. Thus, the anti-inflammatory activity of E. arabicus can be attributed, at least partly, to its anti-oxidant, anti-inflammatory and anti-chemotactic properties.
阿拉伯茶（菊科）生长在阿拉伯半岛。其地上部分具有针对多种炎症病症的民间医学应用。
评估阿拉伯茶（E. arabicus）有机提取物及其主要多甲氧基黄酮的抗炎活性。
通过评估 LD 来评估阿拉伯茶全提取物的急性毒性。通过在足底区域注射角叉菜胶来评估体内抗炎活性。评估爪肿胀体积、组织学变化以及大鼠的爬梯和运动能力。通过角叉菜胶刺激外周血单核细胞 (PBMC) 评估分离化合物的体外抗炎活性。在细胞裂解物和收集的培养基中评估炎症标志物。
提取物被发现是安全的，被认为是未分类的，在大鼠中口服 LD > 2000 mg/kg。以 100 和 250 mg/kg 的剂量对大鼠预先用阿拉伯茶的总提取物处理，可显著抑制角叉菜胶诱导的爪肿胀体积增加和组织病理学变化。此外，它还显著改善了攀爬和运动能力的降低。植物化学研究导致了五种多甲氧基黄酮的分离和鉴定。在角叉菜胶刺激的外周血单核细胞 (PBMC) 中评估了分离化合物的抗炎特性。所有化合物均表现出相当的抗氧化活性。进一步，在用细胞预孵育分离的代谢物后，可显著改善由角叉菜胶刺激引起的环加氧酶-2 (COX-2)、诱导型一氧化氮合酶 (iNOS) 和单核细胞趋化蛋白-1 (MCP-1) 的升高。此外，这些化合物抑制了刺激细胞中收集的培养基中白细胞介素-1β (IL-1β)、白细胞介素-6 (IL-6) 和髓过氧化物酶 (MPO) 的渗漏。
阿拉伯茶在角叉菜胶模型中表现出体内抗炎作用，因为它改善了大鼠爪肿胀、组织病理学变化和运动功能障碍。分离化合物的体外活性在刺激的 PBMC 中得到证实。因此，阿拉伯茶的抗炎活性至少部分归因于其抗氧化、抗炎和趋化作用。
