Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China.
Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China.
Carbohydr Polym. 2020 Jan 1;227:115280. doi: 10.1016/j.carbpol.2019.115280. Epub 2019 Sep 14.
A water-soluble polysaccharide from Monostroma nitidum, designated MWS, was isolated using water extraction, anion-exchange and size-exclusion chromatography. MWS was a sulfated glucuronorhamnan consisting of →3)-α-l-Rhap-(1→, →4)-β-d-GlcpA-(1→ and →2)-α-l-Rhap-(1→ units. Sulfate ester groups located at C-4/C-2 of →3)-α-l-Rhap-(1→ and C-4/C-3 of →2)-α-l-Rhap-(1→ units. In in vitro tests, it was proved that MWS possessed broad spectrum against different viruses, especially for enterovirus 71 (EV71) with nearly no toxicity in relation to cell lines used. MWS may largely inhibit EV71 infection before or during viral adsorption through binding to virus particles and block some early steps of virus life cycle by down-regulating host phosphoinositide 3-kinase /protein kinase B signaling pathway. Intramuscular injection of MWS markedly reduced viral titers in EV71-infected mice. The data demonstrated that MWS could have great promising to become an antiviral drug for prevention and therapy of EV71 infection.
从江蓠属中分离到一种水溶性多糖，命名为 MWS，采用水提、阴离子交换和凝胶过滤色谱法分离。MWS 是一种硫酸化的葡醛酸岩藻聚糖，由→3）-α-l-Rhap-（1→、→4）-β-d-GlcpA-（1→和→2）-α-l-Rhap-（1→单元组成。硫酸酯基团位于→3）-α-l-Rhap-（1→和→2）-α-l-Rhap-（1→单元的 C-4/C-2 和 C-4/C-3 上。在体外试验中，证明 MWS 对不同病毒具有广谱抗病毒活性，特别是对肠道病毒 71（EV71）几乎没有细胞毒性。MWS 可能通过与病毒颗粒结合，在病毒吸附前或吸附过程中大量抑制 EV71 感染，并通过下调宿主磷酯酰肌醇 3-激酶/蛋白激酶 B 信号通路，阻断病毒生命周期的某些早期步骤，从而显著抑制 EV71 感染。肌肉注射 MWS 可显著降低 EV71 感染小鼠的病毒滴度。数据表明，MWS 有望成为预防和治疗 EV71 感染的抗病毒药物。
