Choi Sun-Il, La Im-Joung, Han Xionggao, Men Xiao, Lee Se-Jeong, Oh Geon, Kwon Hee-Yeon, Kim Yong-Deok, Seong Geum-Su, Kim Seung-Hyung, Lee Ok-Hwan
Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Korea.
Atomy R&D Center, 3526, Charyeong-ro, Jeongan-myeon, Gongju-si 32511, Korea.
Foods. 2022 Jun 28;11(13):1925. doi: 10.3390/foods11131925.
This study aims to determine the immunomodulatory effects of a polysaccharide fraction from fermented L. (FMP) in RAW 264.7 macrophages and Balb/c mice. was fermented for 72 h using polysaccharides were extracted using ethanol precipitation. The RAW 264.7 cells exposed to FMP (50, 100, and 200 μg/mL) for 24 h showed increased NO production, proinflammatory cytokine (IL-1β, IL-6, and TNF-α) release, and COX-2 and iNOS protein expression. FMP (100, 200 mg/kg) and deacetylasperulosidic acid (DAA) (20 mg/kg) administered orally to Balb/c mice for 14 days upregulated NO production and NK cytotoxicity in abdominal cavity and spleen, respectively. Th1 and Th2 cytokines production and immune cell numbers increased in spleen, mesenteric lymph nodes (MLN), peritoneal exudate cells (PEC), Peyer's patches (PP), and peripheral blood mononuclear cells (PBMC). Therefore, FMP containing DAA can be used as materials for health functional foods to enhance immune responses.
本研究旨在确定发酵罗伊氏乳杆菌多糖级分（FMP）对RAW 264.7巨噬细胞和Balb/c小鼠的免疫调节作用。使用[具体物质]发酵72小时，采用乙醇沉淀法提取多糖。RAW 264.7细胞暴露于FMP（50、100和200μg/mL）24小时后，一氧化氮（NO）生成增加，促炎细胞因子（IL-1β、IL-6和TNF-α）释放增加，环氧化酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）蛋白表达增加。给Balb/c小鼠口服FMP（100、200mg/kg）和去乙酰车叶草苷酸（DAA）（20mg/kg）14天，分别上调腹腔和脾脏中的NO生成及自然杀伤细胞（NK）细胞毒性。脾脏、肠系膜淋巴结（MLN）、腹腔渗出细胞（PEC）、派伊尔结（PP）和外周血单个核细胞（PBMC）中Th1和Th2细胞因子生成及免疫细胞数量增加。因此，含有DAA的FMP可作为健康功能食品的原料以增强免疫反应。
