School of Agriculture and biology, Shanghai Jiao Tong University, Minghang200240, Shanghai, People's Republic of China.
Hangzhou Tea Research Institute, China COOP, Hangzhou City, 310012 Zhejiang, People's Republic of China.
Carbohydr Polym. 2020 Jan 1;227:115362. doi: 10.1016/j.carbpol.2019.115362. Epub 2019 Sep 22.
A new polysaccharide (pFSP) was first isolated from the originally discarded fibrous roots part of Chinese traditional herb, Bletilla striata. pFSP was composed of D-glucose, D-galactose and D-mannose in a molar ratio of 1: 2.03: 3.45 with molecular weight of 9.1 × 10 Da. It could effectively scavenge DPPH and superoxide radicals with inhibition rate of 64.47% and 72.27% at 5.0 mg/ml, higher than that of polysaccharide from Bletilla striata tuber. Structural investigations of the periodate oxidation studies and Smith-degradation as well as the FT-IR spectroscopy were performed, and combined with 1D and 2D NMR spectroscopy, the repeating unit of pFSP contained (1→4)-linked-α-D-Glcp, (1→4)-linked-β-D-Manp and (1→3,6)-linked-β-D-Manp units, together with the branches of (1→6)-linked-β-D-Galp and terminated with (1→)-linked-β-D-Manp residue.
一种新的多糖（pFSP）首先从中国传统草药白芨最初丢弃的纤维状根部部分中分离出来。pFSP 由 D-葡萄糖、D-半乳糖和 D-甘露糖以摩尔比 1:2.03:3.45 组成，分子量为 9.1×10 Da。它可以有效地清除 DPPH 和超氧自由基，在 5.0 mg/ml 时抑制率分别为 64.47%和 72.27%，高于白芨块茎多糖。进行了高碘酸氧化研究、Smith 降解以及傅里叶变换红外光谱分析，并结合 1D 和 2D NMR 光谱，pFSP 的重复单元含有（1→4）-连接的-α-D-Glcp、（1→4）-连接的-β-D-Manp 和（1→3,6）-连接的-β-D-Manp 单元，以及（1→6）-连接的-β-D-Galp 分支，并以（1→）-连接的-β-D-Manp 残基结尾。
