Dunstan R A, Rosse W F
Transfusion. 1985 May-Jun;25(3):219-22. doi: 10.1046/j.1537-2995.1985.25385219901.x.
A case of posttransfusion purpura is reported in which the laboratory determination of the specificity of the causative antibody was initially confused by the presence in the patient's serum of HLA-directed antibodies. The patient was a multiparous 65-year-old woman with a previous history of blood transfusion. She developed the typical clinical features of posttransfusion purpura 8 days following the transfusion of 6 units of packed red cells. The patient was P1A1 negative, and her serum reacted strongly in a radiolabeled monoclonal antibody assay with both P1A1-positive and -negative platelets. Radioimmunoprecipitation demonstrated that the patient's serum contained antibodies both of anti-P1A1 and anti-HLA specificity. Western blotting of normal platelets incubated with the patient's serum verified that the anti-P1A1 antibody was directed against platelet membrane glycoprotein III.
本文报告了一例输血后紫癜病例，患者血清中存在针对HLA的抗体，最初使致病抗体特异性的实验室测定结果产生混淆。患者为一名65岁经产妇，既往有输血史。在输注6单位浓缩红细胞8天后，她出现了输血后紫癜的典型临床特征。患者为P1A1阴性，其血清在放射性标记单克隆抗体检测中与P1A1阳性和阴性血小板均发生强烈反应。放射免疫沉淀显示患者血清中同时含有抗P1A1和抗HLA特异性抗体。用患者血清孵育正常血小板进行的蛋白质印迹分析证实，抗P1A1抗体针对血小板膜糖蛋白III。
