Gerber H, Späth P J, Perret B A, Burckhardt J J
Central Laboratory, Blood Transfusion Service SRC, Berne, Switzerland.
Blut. 1989 Jul;59(1):61-6. doi: 10.1007/BF00320251.
The aim of this laboratory workshop was to evaluate the state of knowledge concerning the demonstration of membrane glycoprotein specific anti-platelet antibodies. The main interest lay in investigating whether specific antibody detection offers possibilities to distinguish the chronic from the acute form of ITP. In five laboratories four different methods were applied to demonstrate such antibodies. These methods are briefly described and compared. In all, except two, of the 45 ITP samples anti-platelet antibodies could be detected by at least one participating laboratory, in 85% of the samples antibodies were found by two or more laboratories. For seven out of eight control samples no positive results were reported. The comparison of glycoprotein specific anti-platelet antibodies shows partly considerable differences which may be due to the different methods as well as the different antibodies used (monoclonal antibody against membrane glycoprotein and antihuman globulin sera). This laboratory workshop leads to the conclusion that by exchange of reagents and patient samples the different methods may be compared and evaluated. The results obtained allowed no further characterization of ITP. All participants agreed on the usefulness of further similar laboratory workshops.
本次实验室研讨班的目的是评估关于膜糖蛋白特异性抗血小板抗体检测的知识水平。主要关注点在于研究特异性抗体检测是否能够区分免疫性血小板减少症（ITP）的慢性和急性形式。在五个实验室中，应用了四种不同方法来检测此类抗体。这些方法将被简要描述并比较。在总共45份ITP样本中，除两份样本外，至少有一个参与实验室能检测到抗血小板抗体，85%的样本被两个或更多实验室检测到抗体。在八份对照样本中，有七份未报告阳性结果。糖蛋白特异性抗血小板抗体的比较显示出部分显著差异，这可能归因于所使用的不同方法以及不同抗体（针对膜糖蛋白的单克隆抗体和抗人球蛋白血清）。本次实验室研讨班得出结论，通过试剂和患者样本的交换，可以比较和评估不同方法。所获得的结果无法对ITP进行进一步特征描述。所有参与者都认同进一步开展类似实验室研讨班的有用性。
