Shibata N, Itoh N, Shigematsu H
Acta Pathol Jpn. 1985 Jan;35(1):9-22.
To evaluate the role of macrophages in glomerulonephritis, the relationship between the existence of macrophages and proteinuria in the long course of glomerulonephritis was studied in accelerated Masugi nephritis in the rat. Non-specific esterase staining was used as a marker of macrophages and the kinetics of glomerular macrophages was analysed by an image processor. Macrophages became detectable 48 hours after nephrotoxic serum injection and their accumulation reached a maximum level at 5 days. At 1 month, they had clearly decreased. At 3 months, however, many macrophages were found within the capsular drop-like lesions and crescents. Urinary protein excretion took roughly the same time course as the number of macrophages for 2 weeks. However, at 1 month, whereas macrophages had decreased, the proteinuria kept a persistently high level. At 3 months it showed a tendency to decrease. The correlation between the rate of appearance of macrophages and the urinary protein excretion assessed in 20 cases exhibited no significance. In addition, discrepancy in their time course was evident at 1 month. Although macrophages may happen to contribute to the tissue injury resulting in proteinuria in the early phase of nephrotoxic serum nephritis, factors other than macrophages should be considered in the subsequent duration of proteinuria in this model.
为了评估巨噬细胞在肾小球肾炎中的作用，在大鼠加速型Masugi肾炎中研究了巨噬细胞的存在与肾小球肾炎病程较长时蛋白尿之间的关系。采用非特异性酯酶染色作为巨噬细胞的标志物，并通过图像处理器分析肾小球巨噬细胞的动力学。在注射肾毒性血清48小时后可检测到巨噬细胞，其聚集在5天时达到最高水平。在1个月时，它们明显减少。然而，在3个月时，在包膜下滴状病变和新月体中发现了许多巨噬细胞。尿蛋白排泄在2周内与巨噬细胞数量的时间进程大致相同。然而，在1个月时，巨噬细胞减少，但蛋白尿持续保持高水平。在3个月时，它有下降的趋势。对20例病例评估的巨噬细胞出现率与尿蛋白排泄之间的相关性无统计学意义。此外，在1个月时它们的时间进程差异明显。虽然在肾毒性血清肾炎的早期阶段巨噬细胞可能碰巧促成了导致蛋白尿的组织损伤，但在该模型中蛋白尿的后续病程中应考虑除巨噬细胞之外的其他因素。
