Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, China.
Int J Biol Sci. 2019 Aug 8;15(10):2156-2169. doi: 10.7150/ijbs.35670. eCollection 2019.
Recently there has been an increasing interest in bioactive factors with robust osteogenic ability and angiogenesis function to repair bone defects. However, previously tested factors have not achieved satisfactory results due to low loading doses and a short protein half-life. Finding a validated stable substitute for these growth factors and apply it to the construction of porous scaffolds with the dual function of osteogenesis and angiogenesis is therefore vital for bone tissue regeneration engineering. Graphene oxide (GO) has attracted increasing attention due to its good biocompatibility, osteogenic, and angiogenic functions. This study aims to design a scaffold composed of mesoporous bioactive glasses (MBG) and GO to investigate whether the composite porous scaffold promotes local angiogenesis and bone healing. Our in vitro studies demonstrate that the MBG-GO scaffolds have better cytocompatibility and higher osteogenesis differentiation ability with rat bone marrow mesenchymal stem cells (rBMSCs) than the purely MBG scaffold. Moreover, MBG-GO scaffolds promote vascular ingrowth and, importantly, enhance bone repair at the defect site in a rat cranial defect model. The new bone was fully integrated not only with the periphery but also with the center of the scaffold. From these results, it is believed that the MBG-GO scaffolds possess excellent osteogenic-angiogenic properties which will make them appealing candidates for repairing bone defects. The novelty of this research is to provide a new material to treat bone defects in the clinic.
最近，人们对具有强大成骨能力和血管生成功能的生物活性因子越来越感兴趣，以修复骨缺损。然而，由于加载剂量低和蛋白质半衰期短，以前测试的因子并未取得令人满意的结果。因此，寻找经过验证的稳定替代品，并将其应用于具有成骨和血管生成双重功能的多孔支架的构建，对于骨组织再生工程至关重要。氧化石墨烯（GO）由于其良好的生物相容性、成骨和血管生成功能而受到越来越多的关注。本研究旨在设计一种由介孔生物活性玻璃（MBG）和 GO 组成的支架，以研究复合多孔支架是否能促进局部血管生成和骨愈合。我们的体外研究表明，与纯 MBG 支架相比，MBG-GO 支架具有更好的细胞相容性和更高的成骨分化能力，与大鼠骨髓间充质干细胞（rBMSCs）共培养。此外，MBG-GO 支架促进血管向内生长，并且在大鼠颅缺损模型中重要的是增强了缺损部位的骨修复。新骨不仅与支架的外围，而且与支架的中心完全整合。从这些结果可以看出，MBG-GO 支架具有优异的成骨-血管生成特性，这将使它们成为修复骨缺损的有吸引力的候选材料。本研究的新颖之处在于为临床治疗骨缺损提供了一种新材料。
