介孔生物活性玻璃支架递送丹酚酸 B 促进大鼠颅骨缺损模型中的骨再生。
Mesoporous Bioactive Glass Scaffold Delivers Salvianolic Acid B to Promote Bone Regeneration in a Rat Cranial Defect Model.
机构信息
Department of Emergency Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, China.
Department of Osteoporosis and Bone Diseases, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.
出版信息
Curr Drug Deliv. 2021;18(3):323-333. doi: 10.2174/1567201817666200916091253.
BACKGROUND
Mesoporous Bioactive Glass (MBG) has been widely studied because of its excellent histocompatibility and degradability. However, due to the lack of good osteoinductive activity, the pure MBG scaffold is not effective in repairing large-scale bone defects.
OBJECTIVE
To observe the repair effect of MBG scaffolds delivering Salvianolic acid B (SB) on critical bone defects in rats.
METHODS
In this study, MBG scaffolds were used as delivery vehicle. SB, a small molecular active drug with good osteogenic differentiation ability, was loaded into the MBG scaffolds at low, medium and high doses. The effect of SB released from the MBG scaffolds on osteogenic differentiation of rat Bone Marrow Mesenchymal Stem Cells (rBMSCs) was investigated using alkaline phosphatase staining, alizarin red staining and real-time quantitative polymerase chain reaction. Moreover, 8 weeks after implantation of the scaffolds, the bone regeneration was evaluated by micro- CT, sequential fluorescence labeling and histological staining analysis.
RESULTS
The in vitro results showed that different doses of SB had similar release rate from scaffolds and could be released from scaffolds continuously. The middle dose (MBG/MSB) and high dose (MBG/HSB) groups significantly promoted the osteogenic differentiation of rBMSCs when compared with a low dose (MBG/LSB) group. Moreover, SB produced significant increases in newly formed bone of calvarial bone defects in rats.
CONCLUSION
It is concluded that the use of MBG scffolds delivering SB is an effective strategy for the treatment of bone defects.
背景
介孔生物活性玻璃（MBG）具有良好的组织相容性和可降解性，因此得到了广泛的研究。然而，由于缺乏良好的成骨活性，纯 MBG 支架在修复大体积骨缺损方面效果不佳。
目的
观察载丹酚酸 B（SB）的 MBG 支架对大鼠临界骨缺损的修复效果。
方法
本研究以 MBG 支架为载体，将具有良好成骨分化能力的小分子活性药物 SB 载入 MBG 支架中，分为低、中、高剂量组。通过碱性磷酸酶染色、茜素红染色和实时定量聚合酶链反应，考察 SB 从 MBG 支架中的释放对大鼠骨髓间充质干细胞（rBMSCs）成骨分化的影响。此外，在支架植入 8 周后，通过 micro-CT、连续荧光标记和组织学染色分析评估骨再生情况。
结果
体外结果表明，不同剂量的 SB 从支架中的释放率相似，均可持续释放。与低剂量组（MBG/LSB）相比，中剂量组（MBG/MSB）和高剂量组（MBG/HSB）明显促进了 rBMSCs 的成骨分化。此外，SB 明显增加了大鼠颅骨骨缺损中新形成的骨量。
结论
综上所述，载 SB 的 MBG 支架的应用是治疗骨缺损的一种有效策略。
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