Department of Biochemistry, Guindy Campus, University of Madras, Chennai, India.
Department of Medical Biochemistry, Taramani Campus, University of Madras, Chennai, India.
J Biochem Mol Toxicol. 2019 Dec;33(12):e22404. doi: 10.1002/jbt.22404. Epub 2019 Oct 8.
Discovering the utmost effective and targeted chemotherapy for hepatocellular carcinoma is still a significant challenge. In the present study, diethylnitrosamine was used as a liver carcinogen and boldine a compound of boldo. We anticipated the hypothesis that boldine endow antiproliferative and promote apoptosis on hepatocarcinoma rats. We analyzed that boldine alters the tumor biomarkers and liver markers enzyme levels. Also, we determined boldine modulate the enzymatic and nonenzymatic antioxidant activities, as well as messenger RNA and protein expressions of Bcl2, Bax, and cleaved caspase 3 by reverse transcription polymerase chain reaction and Western blot analysis, respectively. It was also manifested by histopathology studies in liver tissues of HCC rats. Our finding suggested that boldine has antioxidant activity, and moreover, also contributes apoptotic nature by upregulating the protein expression of Bax, and cleaved caspase 3. Our data accomplishes that boldine a candidate drug has dynamic therapeutic activity and suitable for the treatment of HCC.
发现最有效和最具针对性的肝癌化疗仍然是一个重大挑战。在本研究中，二乙基亚硝胺被用作肝癌致癌物，而 Boldine 是 Boldo 的一种化合物。我们假设 Boldine 对肝癌大鼠具有抗增殖和促进凋亡的作用。我们分析了 Boldine 改变肿瘤生物标志物和肝脏标志物酶水平的情况。此外，我们通过逆转录聚合酶链反应和 Western blot 分析分别确定了 Boldine 对酶和非酶抗氧化活性以及 Bcl2、Bax 和 cleaved caspase 3 的信使 RNA 和蛋白表达的调节作用。这也通过 HCC 大鼠肝组织的组织病理学研究得到了证实。我们的发现表明，Boldine 具有抗氧化活性，并且通过上调 Bax 和 cleaved caspase 3 的蛋白表达，还具有促进凋亡的特性。我们的数据表明，Boldine 是一种有潜力的药物，具有动态治疗活性，适合治疗 HCC。
