Wei Ying, Yi Jin-Ke, Chen Jun, Huang Huimin, Wu Lun, Yin Xufeng, Wang Jinjin
Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, China.
Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Rehabilitation department, Shiyan, Hubei 442008, China.
J Trace Elem Med Biol. 2022 Dec;74:127052. doi: 10.1016/j.jtemb.2022.127052. Epub 2022 Aug 3.
Reactive oxygen species (ROS) regulate various cellular signaling pathways and play an important role in the occurrence and development of hepatocellular carcinoma (HCC). Excessive accumulation of ROS can promote HCC. Trace element boron has a wide range of biological effects, including anti-oxidation, anti-tumor, immune regulation and so on.
In this study, we investigated the anticancer effects of Sodium tetraborate decahydrate (NaB) in improving oxidative stress and regulating apoptosis in mouse HCC. HCC was induced by intraperitoneal injection of diethylnitrosamine (DEN) 25 mg/kg once at the age of 2 weeks and 100 mg/kg again at the age of 6 weeks in healthy C3H/HeN male mice. At 8 weeks of age, different concentrations of NaB were given intragastric treatment once a day for 20 weeks. Oxidative stress markers, antioxidant status and liver enzyme analysis were detected to evaluate the effectiveness of NaB in inhibiting cancer induction. The anticancer properties of NaB were confirmed by observing the liver index and morphology, and analyzing the expression of apoptotic genes and proteins. Our results showed that boron significantly reduced the production of ROS, and down-regulated the expression of the anti-apoptotic protein Bcl2 and up-regulated the expression of the pro-apoptotic proteins P53, Bax, and caspase 3.
Boron has great potential to reduce the effects of oxidative stress, which may help it inhibit the progression of HCC.
活性氧（ROS）调节多种细胞信号通路，在肝细胞癌（HCC）的发生发展中起重要作用。ROS的过度积累可促进肝癌发生。微量元素硼具有广泛的生物学效应，包括抗氧化、抗肿瘤、免疫调节等。
在本研究中，我们研究了十水合四硼酸钠（NaB）在改善小鼠肝癌氧化应激和调节细胞凋亡方面的抗癌作用。通过在2周龄时腹腔注射25mg/kg二乙基亚硝胺（DEN）一次，6周龄时再次注射100mg/kg，诱导健康C3H/HeN雄性小鼠发生肝癌。8周龄时，给予不同浓度的NaB灌胃治疗，每天一次，共20周。检测氧化应激标志物、抗氧化状态和肝酶分析，以评估NaB抑制癌症诱导的有效性。通过观察肝脏指数和形态，并分析凋亡基因和蛋白质的表达，证实了NaB的抗癌特性。我们的结果表明，硼显著降低了ROS的产生，下调了抗凋亡蛋白Bcl2的表达，上调了促凋亡蛋白P53、Bax和caspase 3的表达。
硼具有极大潜力减轻氧化应激的影响，这可能有助于其抑制肝癌进展。
