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[用于鉴定阴道毛滴虫的新型荧光显微镜技术]

[New fluorescence microscopic technic for the identification of Trichomonas vaginalis].

作者信息

Grossgebauer K, Kegel M

出版信息

Hautarzt. 1979 Aug;30(8):434-6.

PMID:315935
Abstract

A new fluorescent dye for the identification of trichomonas vaginalis is described. Specimens under investigation were mixed with 4',6-diamidino-2-phenylindol (Dapi) and examined using white light and UV-light. The combined use of the two techniques allows to recognize the mobile non fluorescing and the dead intensive yellow fluorescing trichomonads. Devitalized epithelial cells, bacteria and leucocytes in contrast to living trichomonas are stained yellow in a few minutes. This is of diagnostic help.

摘要

描述了一种用于鉴定阴道毛滴虫的新型荧光染料。将受检标本与4',6-二脒基-2-苯基吲哚(DAPI)混合,并用白光和紫外光进行检查。两种技术联合使用可识别活动的无荧光和死亡的强烈黄色荧光的滴虫。与活的滴虫相比,失活的上皮细胞、细菌和白细胞在几分钟内被染成黄色。这有助于诊断。

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