Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China.
Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China.
Int J Biol Macromol. 2020 Jan 1;142:366-375. doi: 10.1016/j.ijbiomac.2019.09.107. Epub 2019 Oct 5.
Osteoinductive bone filling biomaterials are in high demand for effective bone defect reconstruction. In this study, we aimed to design both organic and inorganic substances containing strontium-doped hydroxyapatite/silk fibroin (SrHA/SF) biocomposite nanospheres as an osteoinductive bone defect-filling biomaterial. SrHA/SF nanospheres were prepared with different concentration of Sr using ultrasonic coprecipitation method. The nanospheres were characterized using XRD, FTIR, SEM, TEM, ICP-AES and TGA. Solid and dense SrHA/SF nanospheres with 500-700 nm size and rough surfaces were synthesized successfully. Higher crystallinity and HA/SF phase were observed with the increase in Sr-concentration. The doping of different concentration of Sr did not affect the size and surface characteristics of the nanospheres. ICP-AES data showed that Sr/Ca ratio in SrHA/SF is very close to the nominal value. Nanospheres with higher concentration of Sr did not negatively affect the biocompatibility, but enhanced viability of mesenchymal stem cells (MSCs). Moreover, SrHA/SF nanospheres showed higher osteogenic differentiation potential compared to HA/SF nanospheres as indicated by the results from ALP staining, ALP activity, and Runx2, Alp, Col-1 and Opn gene expression assay in MSCs culture. Our findings suggest this novel design of biocompatible and osteoinductive SrHA/SF biocomposite nanospheres as a potential bone defect-filling biomaterial for bone regenerative applications.
具有骨诱导性的骨填充生物材料对于有效的骨缺损重建有很高的需求。在这项研究中，我们旨在设计含有锶掺杂羟基磷灰石/丝素（SrHA/SF）生物复合材料纳米球的有机和无机物质，将其作为一种具有骨诱导性的骨缺损填充生物材料。我们采用超声共沉淀法，用不同浓度的 Sr 来制备 SrHA/SF 纳米球。我们使用 XRD、FTIR、SEM、TEM、ICP-AES 和 TGA 对纳米球进行了表征。我们成功合成了尺寸为 500-700nm、表面粗糙的具有固态和高密度的 SrHA/SF 纳米球。随着 Sr 浓度的增加，观察到更高的结晶度和 HA/SF 相。不同浓度 Sr 的掺杂并未影响纳米球的尺寸和表面特性。ICP-AES 数据表明，SrHA/SF 中的 Sr/Ca 比值非常接近标称值。较高浓度 Sr 的纳米球并没有对细胞的生物相容性产生负面影响，反而增强了间充质干细胞（MSCs）的活力。此外，与 HA/SF 纳米球相比，SrHA/SF 纳米球在碱性磷酸酶（ALP）染色、ALP 活性、以及 Runx2、Alp、Col-1 和 Opn 基因表达检测结果中，表现出更高的成骨分化潜能，这表明在 MSC 培养中，SrHA/SF 生物复合材料纳米球具有良好的生物相容性和骨诱导性。我们的研究结果表明，这种新型的生物相容性和骨诱导性 SrHA/SF 生物复合材料纳米球具有作为骨再生应用中潜在的骨缺损填充生物材料的潜力。
