Department of Clinical Lab Science, Arkansas State University, AR 72467, USA.
Department of Physical Therapy, P.O. Box 910, Arkansas State University, AR 72467, USA.
Int J Antimicrob Agents. 2020 Jan;55(1):105818. doi: 10.1016/j.ijantimicag.2019.09.022. Epub 2019 Oct 5.
Campylobacter jejuni is a Gram-negative, microaerophilic bacteria that is the leading cause of food-borne illnesses in the United States; it frequently causes severe diarrhoea. Campylobacter jejuni has recently demonstrated antibiotic resistance; therefore, alternative methods for treating these infections must be investigated. Visible light has proven to be an effective inhibitory agent for many medically significant species of bacteria and fungi.
Visible light in the blue range (405 nm and 464 nm) was administered to a suspension of Campylobacter jejuni. Brain-heart infusion agar was inoculated with the suspension of the organism following exposure to blue light at a dose of 24 Jcm for each wavelength. Inoculated plates were placed into a traditional Campy jar in order to mimic a 5% O atmosphere and incubated at 37 °C for 48 hours. Following incubation, colony forming units were counted and compared with controls to determine if the light energy had effectively inhibited the growth of the organism.
Both 405 nm and 464 nm light at a dose of 24 Jcm were shown to be effective inhibitors of Campylobacter jejuni, with kill rates ranging from 82.4-98.9%.
Blue light effectively inhibits Campylobacter jejuni.
空肠弯曲菌是一种革兰氏阴性、微需氧细菌，是美国食源性疾病的主要致病菌；它经常引起严重腹泻。空肠弯曲菌最近表现出抗生素耐药性；因此，必须研究治疗这些感染的替代方法。可见光已被证明对许多具有重要医学意义的细菌和真菌是一种有效的抑制物。
将 405nm 和 464nm 的可见光照射到空肠弯曲菌的悬浮液中。在每种波长的 24J/cm 剂量下暴露于蓝光后，将悬浮液接种到脑心浸液琼脂上。将接种平板放入传统的弯曲菌罐中，以模拟 5%O 的气氛，并在 37°C 下孵育 48 小时。孵育后，计数菌落形成单位，并与对照进行比较，以确定光能是否有效抑制了该生物体的生长。
405nm 和 464nm 的光在 24J/cm 的剂量下均被证明是空肠弯曲菌的有效抑制剂，杀灭率范围为 82.4-98.9%。
蓝光能有效抑制空肠弯曲菌。
