Wang Xin, Niu Ye, Yue Chen-Xi, Fu Shuang, Wang Rui-Tao
Department of Internal Medicine, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang, 150081, China.
Department of Geriatrics, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, 150086, China.
J Psychiatr Res. 2019 Dec;119:102-106. doi: 10.1016/j.jpsychires.2019.10.002. Epub 2019 Oct 3.
Enterocyte damage and subsequent microbial translocation drive neuroinflammation in the pathogenesis of Alzheimer's disease (AD). Human ileal bile acid binding protein (I-BABP) and intestinal fatty acid binding proteins (I-FABP) are the indicators of enterocyte damage. Lipopolysaccharide-binding protein (LBP) is an indirect marker of microbial translocation. The activation of peripheral innate immune cells plays a crucial role in modulating AD progression. Galectin-9 is a versatile immunomodulatory molecule. The purpose of this study was to determine I-FABP, I-BABP, LBP, and galectin-9 levels in MCI and AD and investigate the relationship between I-FABP, I-BABP, LBP and galectin-9. In this study, I-FABP, I-BABP, LBP, and galectin-9 levels were measured using ELISA assay in 115 AD patients, 115 MCI patients, and 115 non-demented control subjects. Increased I-BABP and galectin-9 were observed in MCI and AD patients. Furthermore, AD patients had higher I-BABP and galectin-9 levels compared with MCI patients. However, I-FABP and LBP in three groups had no difference. I-BABP levels were positively correlated with galectin-9, after adjusting confounding factors (r = 0.409, p < 0.001). In addition, multivariate analysis revealed that increased I-BABP and galectin-9 levels were significantly associated with reduced mini-mental state examination (MMSE) score. In conclusion, galectin-9 is correlated with I-BABP after adjusting confounding covariates. Moreover, increased I-BABP and galectin-9 in MCI and AD are significant factors for reduced MMSE score. Further studies are needed.
肠上皮细胞损伤及随后的微生物易位在阿尔茨海默病（AD）发病机制中驱动神经炎症。人回肠胆汁酸结合蛋白（I-BABP）和肠脂肪酸结合蛋白（I-FABP）是肠上皮细胞损伤的指标。脂多糖结合蛋白（LBP）是微生物易位的间接标志物。外周固有免疫细胞的激活在调节AD进展中起关键作用。半乳糖凝集素-9是一种多功能免疫调节分子。本研究的目的是测定轻度认知障碍（MCI）和AD患者的I-FABP、I-BABP、LBP和半乳糖凝集素-9水平，并研究I-FABP、I-BABP、LBP与半乳糖凝集素-9之间的关系。在本研究中，采用酶联免疫吸附测定法（ELISA）检测了115例AD患者、115例MCI患者和115例非痴呆对照者的I-FABP、I-BABP、LBP和半乳糖凝集素-9水平。在MCI和AD患者中观察到I-BABP和半乳糖凝集素-9升高。此外，与MCI患者相比，AD患者的I-BABP和半乳糖凝集素-9水平更高。然而，三组中的I-FABP和LBP没有差异。在调整混杂因素后，I-BABP水平与半乳糖凝集素-9呈正相关（r = 0.409，p < 0.001）。此外，多因素分析显示，I-BABP和半乳糖凝集素-9水平升高与简易精神状态检查表（MMSE）评分降低显著相关。总之，在调整混杂协变量后，半乳糖凝集素-9与I-BABP相关。此外，MCI和AD中I-BABP和半乳糖凝集素-9升高是MMSE评分降低的重要因素。需要进一步研究。
