测量 CAT 酶的表达和活性，以确定大豆的耐铝性。
Measuring the expression and activity of the CAT enzyme to determine Al resistance in soybean.
机构信息
Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China.
Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Chenggong, Kunming, 650500, China.
出版信息
Plant Physiol Biochem. 2019 Nov;144:254-263. doi: 10.1016/j.plaphy.2019.09.026. Epub 2019 Sep 17.
To elucidate the mechanism of soybean resistance to Al, physiological and biochemical indices and antioxidant enzyme expression and activities were systematically analyzed in Al-sensitive (Glycine max Merr., Yunnan Province of China, SB) and Al-resistant Dambo (Glycine max Merr., Kyoto of Japan, RB) black soybean plants. According to the results, the contents of hydrogen peroxide (HO) and malondialdehyde (MDA) in RB root tips were significantly lower than those in SB root tips, though the opposite results occurred for soluble protein contents. Moreover, the expression and activities of superoxide dismutase (SOD, EC), peroxidase (POD, EC) and catalase (CAT, EC) under 0-400 μM Al for 0-96 h were greater in RB than in SB. However, below 100 μM Al, the activities of those enzymes in SB increased with increasing Al concentration and treatment duration, with SOD activity being lowest and CAT activity exceeding that of POD with increasing Al concentration. Overall, enzyme activity in SB treated with Al at concentrations greater than 200 μM was lower than that in the SB control (CK; not treated with Al) and decreased with treatment duration. Additionally, at Al concentrations lower than 200 μM, enzyme activities in RB were significantly greater than those in RB CK and increased with both Al concentration and treatment duration. Moreover, enzyme activity in RB treated with 400 μM Al was slightly greater than that in RB CK. Thus, CAT activity determines soybean resistance to Al. These results indicate that soybean resistance to Al can be enhanced by regulating the expression and activity of antioxidant enzymes to remove HO under Al stress.
为了阐明大豆对铝的抗性机制，本研究系统分析了铝敏感品种（中国云南省的 Glycine max Merr.，SB）和铝抗性品种（日本京都的 Glycine max Merr.，RB）黑大豆植株的生理生化指标和抗氧化酶表达及活性。结果表明，RB 根尖端的过氧化氢（HO）和丙二醛（MDA）含量明显低于 SB 根尖端，而可溶性蛋白含量则相反。此外，在 0-400 μM Al 处理 0-96 h 期间，0-400 μM Al 下 RB 的超氧化物歧化酶（SOD，EC）、过氧化物酶（POD，EC）和过氧化氢酶（CAT，EC）的表达和活性均大于 SB。然而，在低于 100 μM Al 时，SB 中这些酶的活性随着 Al 浓度和处理时间的增加而增加，SOD 活性最低，随着 Al 浓度的增加，CAT 活性超过了 POD 活性。总体而言，在 Al 浓度大于 200 μM 的条件下处理 SB 后，其酶活性低于 SB 对照（未用 Al 处理），并随着处理时间的延长而降低。此外，在低于 200 μM Al 浓度时，RB 中的酶活性显著高于 RB CK，并且随着 Al 浓度和处理时间的增加而增加。此外，在 400 μM Al 处理下，RB 的酶活性略高于 RB CK。因此，CAT 活性决定了大豆对 Al 的抗性。这些结果表明，通过调节抗氧化酶的表达和活性来清除 Al 胁迫下的 HO，可以增强大豆对 Al 的抗性。
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