Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 460-8601, Japan.
School of Agriculture, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 460-8601, Japan.
Biochem Biophys Res Commun. 2019 Dec 3;520(2):291-296. doi: 10.1016/j.bbrc.2019.09.143. Epub 2019 Oct 5.
Cis-prenyltransferases are enzymes responsible for the biosynthesis of glycosyl carrier lipids, natural rubber, and some secondary metabolites. Certain organisms, including some archaeal species, possess multiple genes encoding cis-prenyltransferase homologs, and the physiological roles of these seemingly-redundant genes are often obscure. Cis-prenyltransferases usually form homomeric complexes, but recent reports have demonstrated that certain eukaryotic enzymes are heteromeric protein complexes consisting of two homologous subunits. In this study, three cis-prenyltransferase homolog proteins, MM_0014, MM_0618, and MM_1083, from the methanogenic archaeon Methanosarcina mazei are overexpressed in Escherichia coli and partially purified for functional characterization. Coexistence of MM_0618 and MM_1083 exhibits prenyltransferase activity, while each of them alone has almost no activity. The chain-lengths of the products of this heteromeric enzyme are in good agreement with those of glycosyl carrier lipids extracted from M. mazei, which are likely di- and tetra-hydrogenated decaprenyl phosphates, suggesting that the MM_0618/MM_1083 heteromer is involved in glycosyl carrier lipid biosynthesis. MM_0014 acts as a typical homomeric cis-prenyltransferase and produces shorter products.
顺式-prenyl 转移酶是负责糖基载体脂质、天然橡胶和一些次生代谢物生物合成的酶。某些生物体，包括一些古菌物种，拥有多个编码顺式-prenyl 转移酶同源物的基因，这些看似冗余的基因的生理作用通常不明确。顺式-prenyl 转移酶通常形成同型复合物，但最近的报告表明，某些真核酶是由两个同源亚基组成的异源蛋白复合物。在这项研究中，来自产甲烷古菌 Methanosarcina mazei 的三种顺式-prenyl 转移酶同源蛋白 MM_0014、MM_0618 和 MM_1083 在大肠杆菌中过表达并进行部分纯化以进行功能表征。MM_0618 和 MM_1083 的共存表现出 prenyltransferase 活性，而它们各自单独几乎没有活性。该异源酶产物的链长与从 M. mazei 中提取的糖基载体脂质的链长非常一致，这些产物可能是二氢和四氢化 decaprenyl 磷酸盐，表明 MM_0618/MM_1083 异源体参与糖基载体脂质生物合成。MM_0014 作为一种典型的同型顺式-prenyl 转移酶，产生较短的产物。
