Department of Biology, Stanford University, Stanford, CA 94305.
HHMI, Stanford University, Stanford, CA 94305.
Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21914-21924. doi: 10.1073/pnas.1911400116. Epub 2019 Oct 8.
Plant cells maintain remarkable developmental plasticity, allowing them to clonally reproduce and to repair tissues following wounding; yet plant cells normally stably maintain consistent identities. Although this capacity was recognized long ago, our mechanistic understanding of the establishment, maintenance, and erasure of cellular identities in plants remains limited. Here, we develop a cell-type-specific reprogramming system that can be probed at the genome-wide scale for alterations in gene expression and histone modifications. We show that relationships among H3K27me3, H3K4me3, and gene expression in single cell types mirror trends from complex tissue, and that H3K27me3 dynamics regulate guard cell identity. Further, upon initiation of reprogramming, guard cells induce H3K27me3-mediated repression of a regulator of wound-induced callus formation, suggesting that cells in intact tissues may have mechanisms to sense and resist inappropriate dedifferentiation. The matched ChIP-sequencing (seq) and RNA-seq datasets created for this analysis also serve as a resource enabling inquiries into the dynamic and global-scale distribution of histone modifications in single cell types in plants.
植物细胞保持着显著的发育可塑性，使它们能够无性繁殖，并在受伤后修复组织；然而，植物细胞通常稳定地保持一致的身份。尽管这种能力很早就被认识到，但我们对植物细胞中细胞类型身份的建立、维持和消除的机制理解仍然有限。在这里，我们开发了一种细胞类型特异性的重编程系统，可以在全基因组范围内探测基因表达和组蛋白修饰的变化。我们表明，单个细胞类型中 H3K27me3、H3K4me3 和基因表达之间的关系反映了复杂组织中的趋势，并且 H3K27me3 动力学调节保卫细胞身份。此外，在重编程开始时，保卫细胞诱导 H3K27me3 介导的对创伤诱导愈伤组织形成调节剂的抑制，表明完整组织中的细胞可能具有感知和抵抗不当去分化的机制。为了进行这项分析，还创建了匹配的 ChIP-seq（seq）和 RNA-seq 数据集，它们也可用作资源，可用于研究单个植物细胞类型中组蛋白修饰的动态和全局分布。
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