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开花过程中拟南芥茎尖分生组织中基因表达和组蛋白标记的时间动态。

Temporal dynamics of gene expression and histone marks at the Arabidopsis shoot meristem during flowering.

机构信息

Department of Molecular Biology, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany.

Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.

出版信息

Nat Commun. 2017 May 17;8:15120. doi: 10.1038/ncomms15120.

DOI:10.1038/ncomms15120
PMID:28513600
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5442315/
Abstract

Plants can produce organs throughout their entire life from pluripotent stem cells located at their growing tip, the shoot apical meristem (SAM). At the time of flowering, the SAM of Arabidopsis thaliana switches fate and starts producing flowers instead of leaves. Correct timing of flowering in part determines reproductive success, and is therefore under environmental and endogenous control. How epigenetic regulation contributes to the floral transition has eluded analysis so far, mostly because of the poor accessibility of the SAM. Here we report the temporal dynamics of the chromatin modifications H3K4me3 and H3K27me3 and their correlation with transcriptional changes at the SAM in response to photoperiod-induced flowering. Emphasizing the importance of tissue-specific epigenomic analyses we detect enrichments of chromatin states in the SAM that were not apparent in whole seedlings. Furthermore, our results suggest that regulation of translation might be involved in adjusting meristem function during the induction of flowering.

摘要

植物可以在其整个生命周期中从位于生长点的多能干细胞中产生器官，这个生长点叫做茎尖分生组织（SAM）。在开花的时候，拟南芥的 SAM 会改变命运，开始产生花朵而不是叶子。开花时间的准确把握在一定程度上决定了繁殖的成功，因此受到环境和内在因素的控制。到目前为止，表观遗传调控如何促进花的转变还不清楚，这主要是因为 SAM 很难接近。在这里，我们报告了染色质修饰 H3K4me3 和 H3K27me3 的时间动态及其与光周期诱导开花时 SAM 中转录变化的相关性。我们强调了组织特异性表观基因组分析的重要性，在整个幼苗中没有明显的情况下，我们检测到了 SAM 中染色质状态的富集。此外，我们的结果表明，在诱导开花过程中，翻译的调节可能参与了调整分生组织的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/e1de25c5a768/ncomms15120-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/23fc1409055b/ncomms15120-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/3bc2c879bb9b/ncomms15120-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/5ff1b54dd1d6/ncomms15120-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/528b20c4a004/ncomms15120-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/e1de25c5a768/ncomms15120-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/23fc1409055b/ncomms15120-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/9d466140deed/ncomms15120-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/d58a1fbd670c/ncomms15120-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/3bc2c879bb9b/ncomms15120-f4.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/528b20c4a004/ncomms15120-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/5442315/e1de25c5a768/ncomms15120-f7.jpg

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