Qingdao University Medical College, 308 Ningxia Road, Qingdao, Shandong 266021, China.
College of Pharmaceutical Sciences, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Southwest University, Chongqing, China.
Food Funct. 2019 Nov 1;10(11):7142-7151. doi: 10.1039/c9fo01891c. Epub 2019 Oct 9.
We report here an acidic polysaccharide, namely RSP-3, which ameliorates acute kidney injury and is obtained from Sanguisorba officinalis. We extracted and purified two polysaccharides from this herb based on the acidity and screened them for their effect in regulating the immunological activity of macrophages. Among them, RSP-3 exhibited significant anti-inflammatory activity against lipopolysaccharide (LPS)-stimulated macrophages by decreasing TNF-α and IL-6 levels. Subsequently, we found that RSP-3 suppressed ER stress, reduced ROS production and blocked NF-κBp65 translocation. After fully characterizing RSP-3 with a series of analytical technologies, we tested its anti-acute kidney injury (AKI) effect in vivo. In a murine AKI model induced by LPS, treatment with RSP-3 effectively ameliorated renal function. Besides, it decreased the levels of TNF-α and IL-6 in serum and reduced macrophage infiltration in injured kidney tissue. In sum, RSP-3, with a significant protective effect against AKI by showing anti-inflammatory activity, may become a meaningful drug candidate for treatment of AKI.
我们在这里报告了一种酸性多糖，即 RSP-3，它可以改善急性肾损伤，并且是从地榆中提取的。我们根据酸度从这种草药中提取并纯化了两种多糖，并筛选了它们对调节巨噬细胞免疫活性的作用。其中，RSP-3 通过降低 TNF-α 和 IL-6 水平，对脂多糖 (LPS) 刺激的巨噬细胞表现出显著的抗炎活性。随后，我们发现 RSP-3 抑制了内质网应激，减少了 ROS 的产生并阻止了 NF-κBp65 的易位。在使用一系列分析技术对 RSP-3 进行充分表征后，我们在体内检测了其抗急性肾损伤 (AKI) 的作用。在 LPS 诱导的小鼠 AKI 模型中，用 RSP-3 治疗可有效改善肾功能。此外，它还降低了血清中 TNF-α 和 IL-6 的水平，并减少了受损肾脏组织中的巨噬细胞浸润。总之，RSP-3 通过发挥抗炎活性对 AKI 具有显著的保护作用，可能成为治疗 AKI 的有意义的药物候选物。
