6-巯基嘌呤处理大鼠比目鱼肌的肌肉萎缩和组织病理学

Muscle atrophy and histopathology of the soleus in 6-mercaptopurine-treated rats.

作者信息

Jaweed M M, Alleva F R, Herbison G J, Ditunno J F, Balazs T

出版信息

Exp Mol Pathol. 1985 Aug;43(1):74-81. doi: 10.1016/0014-4800(85)90056-5.

Abstract

This study was conducted to examine the histochemical changes occurring due to neonatal 6-mercaptopurine (6-MP) treatment (2 mg/kg, sc, between 2 and 22 days after birth) in the slow-twitch muscle (soleus) of adult male and female rats. At 6 months of age, the control and the 6-MP-treated rats were evaluated for the sciatic nerve conduction to the soleus and for the soleus atrophy and histopathology of the type I (slow-twitch) and type II (fast-twitch) muscle fibers. Compared to the normal controls, the 6-MP-treated male and female rats showed similar sciatic nerve conduction to the soleus. However, there was a significant muscle atrophy (57-60%, P less than 0.01) and a decrease in fiber areas of the type I (42-54%, P less than 0.05) and type II (41-71%, P less than 0.01) fibers. The number of type II fibers declined significantly (7.4-14.8%, P less than 0.05). It is proposed that the soleus muscle atrophy and histopathology in 6-MP-treated rats is unrelated to nerve conduction defects and may be related to growth inhibition caused by an interference of the drug during normal differentiation of muscle fibers.

摘要

本研究旨在检测成年雄性和雌性大鼠慢肌（比目鱼肌）因新生期6-巯基嘌呤（6-MP）处理（出生后2至22天，皮下注射2mg/kg）而发生的组织化学变化。在6月龄时，对对照组和6-MP处理组大鼠进行评估，检测坐骨神经向比目鱼肌的传导情况、比目鱼肌萎缩情况以及I型（慢肌）和II型（快肌）肌纤维的组织病理学变化。与正常对照组相比，6-MP处理的雄性和雌性大鼠坐骨神经向比目鱼肌的传导情况相似。然而，出现了显著的肌肉萎缩（57 - 60%，P < 0.01），I型肌纤维（42 - 54%，P < 0.05）和II型肌纤维（41 - 71%，P < 0.01）的纤维面积减小。II型肌纤维数量显著减少（7.4 - 14.8%，P < 0.05）。研究表明，6-MP处理组大鼠比目鱼肌萎缩和组织病理学变化与神经传导缺陷无关，可能与药物在肌纤维正常分化过程中的干扰导致生长抑制有关。