Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan, P. R. China.
Criminal Investigation Team of Yueyang Public Security Bureau, Yueyang, Hunan, P. R. China.
Electrophoresis. 2019 Dec;40(23-24):3099-3107. doi: 10.1002/elps.201900221. Epub 2019 Oct 24.
The Microreader™ 20A ID system is designed for forensic applications such as personal identification, parentage testing, and research. It includes 13 combined DNA index system (CODIS) short tandem repeat (STR) loci (CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11), three expanded CODIS STR loci (D12S391, D19S433, and D2S1338), three non-CODIS STR loci (D6S1043, Penta D, and Penta E), and the amelogenin locus in one reaction with a six-dye fluorescent (FAM, HEX, TAMAR, ROX, PUR, and QD550) analysis system. In this study, the Microreader™ 20A ID system was validated according to the Scientific Working Group on DNA Analysis Methods validation guidelines for forensic DNA Analysis methods and Chinese national standard, including PCR-based studies, sensitivity study, precision, and accuracy evaluation, stutter calculation, inhibitor tests, species specificity, and DNA mixture studies. Our results suggest that the Microreader™ 20A ID system is a useful tool for personal identification and parentage testing.
Microreader™ 20A ID 系统专为法医应用而设计，例如个人识别、亲子关系测试和研究。它包含 13 个联合 DNA 索引系统 (CODIS) 短串联重复 (STR) 基因座（CSF1PO、FGA、TH01、TPOX、vWA、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51 和 D21S11）、3 个扩展 CODIS STR 基因座（D12S391、D19S433 和 D2S1338）、3 个非 CODIS STR 基因座（D6S1043、Penta D 和 Penta E）以及一个反应中的 amelogenin 基因座，使用六染料荧光（FAM、HEX、TAMAR、ROX、PUR 和 QD550）分析系统。在这项研究中，Microreader™ 20A ID 系统根据法医 DNA 分析方法科学工作小组验证指南和中国国家标准进行了验证，包括基于 PCR 的研究、灵敏度研究、精密度和准确度评估、突跳计算、抑制剂测试、种属特异性和 DNA 混合物研究。我们的结果表明，Microreader™ 20A ID 系统是个人识别和亲子关系测试的有用工具。
