Li Jienan, Luo Haibo, Song Feng, Zhang Lushun, Deng Chuncao, Yu Zailiang, Gao Tianzhen, Liao Miao, Hou Yiping
Department of Forensic Genetics, West China School of Pre-clinical and Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu 610041, Sichuan, China.
Department of Pathology and Pathophysiology, Chengdu Medical College, Chengdu 610500, Sichuan, China.
Forensic Sci Int Genet. 2017 Mar;27:67-73. doi: 10.1016/j.fsigen.2016.12.005. Epub 2016 Dec 12.
Microreader™ 23sp ID system is a new 23-plex STR genotyping system that amplified 21 non-CODIS STR loci (D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290 and D5S2500), one CODIS STR locus (D16S539) and the amelogenin locus in one reaction. Microreader™ 23sp ID system was validated according to the guidelines of "Validation Guidelines for DNA Analysis Methods (2012)" described by the Scientific Working Group on DNA Analysis Methods (SWGDAM), including PCR-based studies, sensitivity study, precision and accuracy evaluation, stutter percentage and peak height ratio, inhibitors, species specificity and DNA mixture studies. Our results suggested that Microreader™ 23sp ID system is a useful tool for identification and parentage testing.
微读™23sp身份识别系统是一种新型的23重短串联重复序列（STR）基因分型系统，可在一次反应中扩增21个非联合DNA索引系统（CODIS）的STR基因座（D6S477、D18S535、D19S253、D15S659、D11S2368、D20S470、D1S1656、D22 - GATA198B05、D7S3048、D8S1132、D4S2366、D21S1270、D13S325、D9S925、D3S3045、D14S608、D10S1435、D12S391、D2S1338、D17S1290和D5S2500）、一个CODIS的STR基因座（D16S539）以及牙釉蛋白基因座。微读™23sp身份识别系统已按照DNA分析方法科学工作组（SWGDAM）发布的《DNA分析方法验证指南（2012年）》进行了验证，包括基于聚合酶链反应（PCR）的研究、灵敏度研究、精密度和准确度评估、拖尾百分比和峰高比、抑制剂、物种特异性以及DNA混合物研究。我们的结果表明，微读™23sp身份识别系统是用于身份鉴定和亲子鉴定的有用工具。
