Dipartimento di Chimica, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Molecules. 2019 Oct 8;24(19):3627. doi: 10.3390/molecules24193627.
Asparagus waste represents products of great interest since many compounds with high biological value are located in the lower portion of the spears. The extraction of bioactive compounds from asparagus by-products is therefore crucial for the purpose of adding value to these by-products. In this paper, bioactive peptides from asparagus waste were extracted, digested, purified and identified. In particular, Alcalase was chosen as the enzyme to use to obtain protein hydrolysate due to its low cost and, consequently, the possibility of implementing the method on a large scale. In order to simplify the peptide extract to reach better identification, the hydrolysate was fractionated by reversed-phase chromatography in 10 fractions. Two tests were carried out for antioxidant activity (ABTS-DPPH) and one for antihypertensive activity (ACE). Fractions with a higher bioactivity score were identified by peptidomics technologies and screened for bioactivity with the use of bioinformatics. For ACE-inhibitor activity, two peptides were synthetized, PDWFLLL and ASQSIWLPGWL, which provided an EC value of 1.76 µmol L and 4.02 µmol L, respectively. For the antioxidant activity, by DPPH assay, MLLFPM exhibited the lowest EC50 value at 4.14 µmol L, followed by FIARNFLLGW and FAPVPFDF with EC50 values of 6.76 µmol L and 10.01 µmol L, respectively. A validation of the five identified peptides was also carried out. The obtained results showed that peptides obtained from asparagus by-products are of interest for their biological activity and are suitable for being used as functional ingredients.
芦笋废料代表了具有很大兴趣的产品，因为许多具有高生物价值的化合物位于芦笋的下部。因此，从芦笋副产物中提取生物活性化合物对于增加这些副产物的价值至关重要。在本文中，从芦笋废料中提取、消化、纯化和鉴定了生物活性肽。特别是，由于成本低，因此选择 Alcalase 作为酶来获得蛋白质水解物，从而有可能在大规模上实施该方法。为了简化肽提取物以达到更好的鉴定，通过反相色谱法将水解物分成 10 个馏分。进行了两项抗氧化活性（ABTS-DPPH）测试和一项降压活性（ACE）测试。通过肽组学技术鉴定具有更高生物活性评分的馏分，并使用生物信息学筛选生物活性。对于 ACE 抑制剂活性，合成了两个肽，PDWFLLL 和 ASQSIWLPGWL，它们的 EC 值分别为 1.76 µmol L 和 4.02 µmol L。对于抗氧化活性，通过 DPPH 测定，MLLFPM 表现出最低的 EC50 值为 4.14 µmol L，其次是 FIARNFLLGW 和 FAPVPFDF，其 EC50 值分别为 6.76 µmol L 和 10.01 µmol L。还对鉴定出的五个肽进行了验证。获得的结果表明，从芦笋副产物中获得的肽因其生物活性而具有吸引力，适合用作功能性成分。
