Department of Analytical Chemistry, University of Alcalá , Ctra. Madrid-Barcelona, Km. 33.600, E-28871 Alcalá de Henares, Madrid, Spain.
J Agric Food Chem. 2015 Feb 11;63(5):1514-20. doi: 10.1021/jf505037p. Epub 2015 Jan 27.
The processing of fruits, such as cherries, is characterized by generating a lot of waste material such as fruit stones, skins, etc. To contribute to environmental sustainability, it is necessary to recover these residues. Cherry stones contain seeds with a significant amount of proteins that are underused and undervalued. The aim of this work was to extract cherry seed proteins, to evaluate the presence of bioactive peptides, and to identify them by mass spectrometry. The digestion of cherry seed proteins was optimized, and three different enzymes were employed: Alcalase, Thermolysin, and Flavourzyme. Peptide extracts obtained by the digestion of the cherry seed protein isolate with Alcalase and Thermolysin yielded the highest antioxidant and antihypertensive capacities. Ultrafiltration of hydrolysates allowed obtaining fractions with high antioxidant and antihypertensive capabilities. HPLC-Q-TOF-MS together with bioinformatics tools enabled one to identify peptides in these fractions.
水果加工，如樱桃，其特点是会产生大量的废料，如果核、果皮等。为了促进环境可持续性，有必要回收这些残留物。樱桃核含有大量未被充分利用和低估的蛋白质种子。本工作旨在提取樱桃种子蛋白，评估生物活性肽的存在，并通过质谱法对其进行鉴定。优化了樱桃种子蛋白的酶解条件，使用了三种不同的酶：碱性蛋白酶、胰蛋白酶和风味蛋白酶。用碱性蛋白酶和胰蛋白酶消化樱桃籽蛋白分离物得到的肽提取物具有最高的抗氧化和降压能力。水解产物的超滤处理可获得具有高抗氧化和降压能力的级分。HPLC-Q-TOF-MS 结合生物信息学工具可用于鉴定这些级分中的肽。
