Cavaliere Chiara, Montone Angela Michela Immacolata, Aita Sara Elsa, Capparelli Rosanna, Cerrato Andrea, Cuomo Paola, Laganà Aldo, Montone Carmela Maria, Piovesana Susy, Capriotti Anna Laura
Department of Chemistry, Università di Roma "La Sapienza", Piazzale Aldo Moro 5, 00185 Rome, Italy.
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, Portici, 80055 Naples, Italy.
Antioxidants (Basel). 2021 May 6;10(5):734. doi: 10.3390/antiox10050734.
Soybeans () are an excellent source of dietary proteins and peptides with potential biological activities, such as antihypertensive, anti-cholesterol, and antioxidant activity; moreover, they could prevent cancer. Also, soy contains all the essential amino acids for nutrition; therefore, it represents an alternative to animal proteins. The goal of this paper was the comprehensive characterization of medium-sized and short peptides (two to four amino acids) obtained from simulated gastrointestinal digestion. Two different analytical approaches were employed for peptide characterization, namely a common peptidomic analysis for medium-sized peptides and a suspect screening analysis for short peptides, employing an inclusion list of exact / values of all possible amino acid combinations. Moreover, fractionation by preparative reversed-phase liquid chromatography was employed to simplify the starting protein hydrolysate. Six fractions were collected and tested for antioxidative activity by an innovative antioxidant assay on human gastric adenocarcinoma AGS cell lines. The two most active fractions (2 and 3) were then characterized by a peptidomic approach and database search, as well as by a suspect screening approach, in order to identify potential antioxidant amino acid sequences. Some of the peptides identified in these two fractions have been already reported in the literature for their antioxidant activity.
大豆是膳食蛋白质和具有潜在生物活性的肽的优质来源，这些生物活性包括抗高血压、抗胆固醇和抗氧化活性；此外，它们还可以预防癌症。而且，大豆含有营养所需的所有必需氨基酸；因此，它是动物蛋白的一种替代品。本文的目的是对模拟胃肠道消化获得的中型和短肽（两到四个氨基酸）进行全面表征。采用了两种不同的分析方法来表征肽，即对中型肽进行常规的肽组学分析，对短肽进行可疑物筛选分析，使用所有可能氨基酸组合的精确/值的包含列表。此外，采用制备型反相液相色谱进行分离，以简化起始蛋白水解物。收集了六个馏分，并通过对人胃腺癌AGS细胞系的创新抗氧化试验测试其抗氧化活性。然后通过肽组学方法和数据库搜索以及可疑物筛选方法对两个活性最高的馏分（2和3）进行表征，以鉴定潜在的抗氧化氨基酸序列。在这两个馏分中鉴定出的一些肽因其抗氧化活性已在文献中有所报道。
