Chan M, Kano K, Milgrom F
J Immunol Methods. 1979;31(1-2):83-91. doi: 10.1016/0022-1759(79)90288-6.
The principle of mixed agglutination with cell cultures was applied to those cells which could not be obtained as monolayer cell cultures. Artificial monolayers of freshly isolated cells from various tissues were prepared in poly-L-lysine-coated wells of a microtiter U-tray. By means of this technique, murine alloantigens, H-2 private specificities and Thy-1 antigens were demonstrated on cells from thymus, brain, spleen, kidney and liver. Ia.2 antigen was detected on splenocytes but not on thymocytes or fibroblast L cells. Evidence was also presented that HLA A and B locus antigens could be demonstrated on human thymocytes. Sensitivity of this test was shown to be at least 100 times higher than that of the standard lymphocytotoxicity test for detection of HLA antigens.
将细胞培养混合凝集原理应用于无法获得单层细胞培养的细胞。在微量滴定U型板的聚-L-赖氨酸包被孔中制备来自各种组织的新鲜分离细胞的人工单层。通过这种技术，在来自胸腺、脑、脾、肾和肝的细胞上证实了小鼠同种异体抗原、H-2私有特异性和Thy-1抗原。在脾细胞上检测到Ia.2抗原，但在胸腺细胞或成纤维细胞L细胞上未检测到。还提供了证据表明人胸腺细胞上可证实HLA A和B位点抗原。该试验的灵敏度显示比检测HLA抗原的标准淋巴细胞毒性试验至少高100倍。
