Warnatz H, Scheiffarth F, Schmeissner R
Clin Exp Immunol. 1975 Aug;21(2):250-8.
Lymph node and spleen cells from mice immunized in vivo to allogeneic of syngeneic liver antigen are cytotoxic for syngeneic liver cells, but not for syngeneic fibroblasts or established liver cell cultures of allogeneic origin. The cytotoxic activity is mainly dependent on T-cell activity, but a non-T-cell-mediated cytotoxicity may also play a role. Lymphocytotoxicity is inhibited by preincubation of the lymphocytes with syngeneic liver antigen, but not with syngeneic kidney homogenate. The liver-specific lymphocytotoxicity corresponds to the in vivo function of lymphocytes in the development of experimental hepatitis. In vitro immunization of lymphocytes in a Mishell-Dutton culture system also induces liver-specific cytotoxicity. The results indicate that the natural tolerance to self antigens can be lost after invivo as well as in vitro immunization. The induction of self-reactivity of lymphocytes in these experiments may be attributed to regulatory mechanisms of the immune reaction at a cellular level.
在体内对同种异体或同基因肝抗原进行免疫的小鼠的淋巴结和脾细胞，对同基因肝细胞具有细胞毒性，但对同基因成纤维细胞或源自同种异体的已建立的肝细胞培养物没有细胞毒性。细胞毒性活性主要依赖于T细胞活性，但非T细胞介导的细胞毒性也可能起作用。淋巴细胞与同基因肝抗原预孵育可抑制淋巴细胞毒性，但与同基因肾匀浆预孵育则不会。肝脏特异性淋巴细胞毒性与淋巴细胞在实验性肝炎发展中的体内功能相对应。在米舍尔-达顿培养系统中对淋巴细胞进行体外免疫也会诱导肝脏特异性细胞毒性。结果表明，在体内和体外免疫后，对自身抗原的天然耐受性可能会丧失。在这些实验中淋巴细胞自身反应性的诱导可能归因于细胞水平上免疫反应的调节机制。
