Programa de Pós-Graduação em Inovação Farmacêutica, Departamento de Ciências Biológicas e da Saúde, Colegiado de Farmácia, Universidade Federal do Amapá, Macapá, Amapá, Brasil.
Laboratório de Pesquisa em Fármacos, Departamento de Ciências Biológicas e da Saúde, Colegiado de Farmácia, Universidade Federal do Amapá, Macapá, Amapá, Brasil.
Hum Exp Toxicol. 2020 Feb;39(2):127-146. doi: 10.1177/0960327119878257. Epub 2019 Oct 9.
Hydroethanolic preparations of is used in the north of Brazil as a female aphrodisiac. Thus, the objective of this study was to evaluate the action of the hydroethanolic extract of (EHFAo) flowers (21.873 and 44.457 mg/kg) and spilanthol (3 mg/kg) administered orally on reproductive performance and effects on the embryonic development of zebrafish F1 generation. It was observed that in the groups in which males and females received EHFAo and spilanthol, the spawning was interrupted, whereas in the groups in which only the females were treated, spawning occurred during the 21 days. Thus, in the histopathological evaluation of the gonads, it was possible to observe that the percentage of mature cells in the spermatozoa and females was significantly reduced. Only the embryo groups in which parental generation was treated with EHFAo showed lethal and teratogenic effects. On the other hand, the parental groups treated with the spilanthol presented only the lethality. Spilanthol and some metabolites showed good oral availability and important toxicological properties. Thus, it is suggested that the treatment of parental generation of zebrafish with EHFAo and spilanthol caused severe changes in the gonads and on fertility. However, on the embryo, the most striking effects in the development were recorded in the groups in which the parental generation was treated with the EHFAo, while the spilanthol influenced the lethality of the embryos.
在巴西北部，用水醇法制备的 被用作女性催情剂。因此，本研究的目的是评估 花的水醇提取物（EHFAo，分别为 21.873 和 44.457mg/kg）和斯皮兰醇（3mg/kg）经口给药对斑马鱼 F1 代生殖性能和胚胎发育的影响。结果表明，在雌雄鱼均接受 EHFAo 和斯皮兰醇处理的组中，产卵被中断，而仅对雌鱼进行处理的组中，在 21 天内产卵。因此，在对性腺进行组织病理学评估时，发现精子和雌鱼中成熟细胞的比例明显降低。只有接受 EHFAo 处理的亲代胚胎组显示出致死和致畸作用。另一方面，用斯皮兰醇处理的亲代组仅表现出致死作用。斯皮兰醇和一些代谢物具有良好的口服生物利用度和重要的毒理学特性。因此，建议用 EHFAo 和斯皮兰醇处理亲代斑马鱼，会导致性腺和生育能力严重受损。然而，在胚胎发育方面，最显著的影响发生在亲代接受 EHFAo 处理的组中，而斯皮兰醇则影响胚胎的致死率。
