Medrano-Padial Concepción, Puerto María, Moreno F Javier, Richard Tristan, Cantos-Villar Emma, Pichardo Silvia
Area of Toxicology, Faculty of Pharmacy, C/Profesor García González n°2, Universidad de Sevilla, 41012 Seville, Spain.
Area of Cellular Biology, Faculty of Biology, Avda. Reina Mercedes s/n, Universidad de Sevilla, 41012 Seville, Spain.
Antioxidants (Basel). 2019 Oct 9;8(10):467. doi: 10.3390/antiox8100467.
The reduction of sulfur dioxide in wine is a consumer's demand, considering the allergic effects that may occur in people who are sensitive to it. Stilbenes are candidates of great interest for this purpose because of their antioxidant/antimicrobial activities and health properties, and also because they are naturally found in the grapevine. In the present study, the in vitro toxicity of an extract from grapevine shoots (with a stilbene richness of 45.4%) was assessed in two human cell lines. Significant damage was observed from 30 μg/mL after 24 h, and 40 µg/mL after 48 h of exposure. Similarly, the ultrastructural study revealed a significant impairment of cell growing. The extract was able to protect cells against an induced oxidative stress at all concentrations studied. In view of the promising results, a more exhaustive toxicological assessment of the extract is needed to confirm the safety of its further use as additive in wine.
考虑到对二氧化硫敏感的人可能会出现过敏反应，降低葡萄酒中的二氧化硫是消费者的需求。由于芪类化合物具有抗氧化/抗菌活性和健康特性，并且它们天然存在于葡萄藤中，因此是实现这一目的的极具吸引力的候选物质。在本研究中，对葡萄嫩枝提取物（芪类化合物含量为45.4%）在两种人类细胞系中的体外毒性进行了评估。暴露24小时后，在30μg/mL浓度下观察到显著损伤，暴露48小时后，在40μg/mL浓度下观察到显著损伤。同样，超微结构研究显示细胞生长受到显著损害。在所研究的所有浓度下，该提取物都能够保护细胞免受诱导的氧化应激。鉴于这些有前景的结果，需要对该提取物进行更详尽的毒理学评估，以确认其作为葡萄酒添加剂进一步使用的安全性。
