Chen Jie, Sun Haipeng, Tang Weisen, Zhou Lin, Xie Xi, Qu Zhan, Chen Mengfei, Wang Shunyao, Yang Ting, Dai Ying, Wang Yongli, Gao Tangjie, Zhou Qiao, Song Zhuo, Liao Mingmei, Liu Weidong
Department of Essential Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, P. R. China.
GeneTalks Biotech Co., Ltd. Changsha, Hunan, 410000, P. R. China.
J Cancer. 2019 Aug 28;10(21):5264-5271. doi: 10.7150/jca.34944. eCollection 2019.
: Detection of aberrant methylated genes in feces has been developed as an early screening method for colorectal cancer. The aim of this study was to probe the methylation status of SEPT9, BMP3, NDRG4, and SDC2 in stool and study whether methylation of these genes is associated with colorectal cancer. : DNAs were isolated and purified from cancerous and non-cancerous stool samples and colorectal cancer tissue. Gene methylation levels were quantified by methylation-specific PCR on SEPT9, BMP3, NDRG4, and SDC2 and analyzed by a diagnostic model. : DNA methylation of SEPT9, NDRG4 and SDC2, but not BMP3, had diagnostic potential for detecting colorectal cancer. Moreover, integration of SEPT9, NDRG4, and SDC2 methylation demonstrated high feasibility for detecting colorectal cancer and adenoma, with better performance on colorectal cancer than adenoma. : The methylation of SEPT9, NDRG4, and SDC2 in stool may be a potential biomarker for early screening of colorectal cancer.
粪便中异常甲基化基因的检测已被开发为结直肠癌的早期筛查方法。本研究的目的是探究粪便中SEPT9、BMP3、NDRG4和SDC2的甲基化状态，并研究这些基因的甲基化是否与结直肠癌相关。：从癌性和非癌性粪便样本以及结直肠癌组织中分离并纯化DNA。通过对SEPT9、BMP3、NDRG4和SDC2进行甲基化特异性PCR定量基因甲基化水平，并通过诊断模型进行分析。：SEPT9、NDRG4和SDC2的DNA甲基化，而非BMP3，具有检测结直肠癌的诊断潜力。此外，SEPT9、NDRG4和SDC2甲基化的整合显示出检测结直肠癌和腺瘤的高可行性，对结直肠癌的检测性能优于腺瘤。：粪便中SEPT9、NDRG4和SDC2的甲基化可能是结直肠癌早期筛查的潜在生物标志物。
