Wang Qian, Ding Yuan, Yang Min, Guo Dong-Qin, Huang Yu, Zhang Chong-Tao, Pang Yi-Fei, Zhou Nong
the Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs,College of Food and Biological Engineering,Chongqing Three Gorges University Chongqing 404120,China College of Pharmacy and Chemistry,Dali University Dali 671000,China.
the Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs,College of Food and Biological Engineering,Chongqing Three Gorges University Chongqing 404120,China.
Zhongguo Zhong Yao Za Zhi. 2019 Aug;44(15):3203-3212. doi: 10.19540/j.cnki.cjcmm.20190527.102.
In order to provide guidance for the protection and utilization of resources,quality control and breeding of improved varieties,we compared the main phenotypic characters and quality of wild and transplanted Paris polyphylla var. yunnanensis collected from different producing areas. Seven phenotypic characters of 33 samples of P. polyphylla var. yunnanensis collected from Yunnan,Guizhou and Sichuan were determined by conventional methods,and the principal component analysis and cluster analysis were used to analyze the diversity of the samples. The parissaponin( polyphyllin Ⅰ,Ⅱ,Ⅵ,Ⅶ) content of the samples were detected by HPLC,and analyzed by cluster analysis. Correlation analysis of the phenotypic characters and the parissaponin content was performed. There were significant differences in seven phenotypic characters between wild and transplanted samples of P. polyphylla var. yunnanensis from different habitats,with high phenotypic diversity and abundant genetic variation. The results of principal component analysis showed that leaf shape index was the main factor of morphological variation of P. polyphylla var. yunnanensis. Cluster analysis showed that the phenotypic characters of wild and transplanted P. polyphylla var. yunnanensis could not be completely separated. The content of saponins in wild and transplanted samples from different habitats was quite different. Saponins content of 93. 94% samples met the criterion of Chinese Pharmacopoeia 2015 edition,and the overall quality was relatively steady. The results of independent sample t-test showed that there was no significant difference of all the active ingredient between wild and transplanted samples,and it couldn't be used to distinguish between wild and transplanted samples. It is the same as the results of cluster analysis. The results of correlation analysis showed that the phenotypic traits of P. polyphylla var. yunnanensis were correlated with its medicine quality,and the total content of saponins was positively correlated with leaf length and leaf shape index( r = 0. 389,0. 441; P<0. 05). Yunnan,Guizhou and Sichuan are suitable for the growth of P. polyphylla var. yunnanensis. And the transplaned P. polyphylla var. yunnanensis can be used as the same as the wild ones completely. The results provide reference for the protection and selective breeding of P. polyphylla var. yunnanensis.
为了给资源保护与利用、质量控制及优良品种选育提供指导，我们比较了采自不同产地的野生及移栽滇重楼的主要表型性状和质量。采用常规方法测定了从云南、贵州和四川采集的33份滇重楼样品的7个表型性状，并运用主成分分析和聚类分析对样品的多样性进行分析。采用高效液相色谱法检测样品中重楼皂苷（重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ）的含量，并进行聚类分析。对表型性状与重楼皂苷含量进行相关性分析。不同生境的野生及移栽滇重楼在7个表型性状上存在显著差异，表型多样性高，遗传变异丰富。主成分分析结果表明，叶形指数是滇重楼形态变异的主要因素。聚类分析表明，野生及移栽滇重楼的表型性状不能完全分开。不同生境的野生及移栽样品中皂苷含量差异较大。93.94%的样品皂苷含量符合《中国药典》2015年版标准，整体质量相对稳定。独立样本t检验结果表明，野生及移栽样品中所有活性成分均无显著差异，不能用于区分野生及移栽样品。这与聚类分析结果一致。相关性分析结果表明，滇重楼的表型性状与其药用品质相关，皂苷总含量与叶长和叶形指数呈正相关（r = 0.389，0.441；P<0.05）。云南、贵州和四川适合滇重楼生长。移栽的滇重楼可完全与野生滇重楼同等使用。研究结果为滇重楼的保护及良种选育提供参考。
