Zhang Yong-Li, Pan Qi-Ming, Zhang Gui-Jie, Liang Dong
College of Pharmacy,Guilin Medical University Guilin 541004,China.
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources,School of Chemistry and Pharmaceutical Sciences,Guangxi Normal University Guilin 541004,China.
Zhongguo Zhong Yao Za Zhi. 2019 Sep;44(17):3738-3744. doi: 10.19540/j.cnki.cjcmm.20190614.201.
Seventeen compounds were isolated from the 95% ethanol extract of the stems and leaves of Sapium discolor by using various chromatographic techniques,including silica gel,Sephadex LH-20,MCI,ODS,and semi-preparative HPLC. Their structures were elucidated as sapiumin F( 1),kadsulignan C( 2),ciwujiatone( 3),ethylbrevifolin carboxylate( 4),7-hydroxy-8-methoxycoumarin( 5),fraxetin( 6),fraxidin( 7),isofraxidin( 8),6,7,8-trimethoxycoumarin( 9),5,6,7,8-tetramethoxycoumarin( 10),8-hydroxy-5,6,7-trimethoxycoumarin( 11),3,3'-di-O-methylellagic acid( 12),3,3',4'-tri-O-methylellagic acid( 13),3'-methoxyellagic acid 4'-O-α-rhamnopyranoside( 14),4,5-didehydro-chebulic acid triethyl ester( 15),ent-kaurane-3-oxo-16α,17-diol( 16),and abscisic acid( 17) by spectroscopic data. Compound 1 is a new compound. Except for compounds 4,11,and 13,the remaining compounds were isolated from this plant for the first time. All the isolates were evaluated for their in vitro antineuroinflammatory activities,and the results showed that compounds 6 and 15 significantly inhibited nitric oxide production in lipopolysaccharide-induced BV-2 microglial cells,with IC50 values of 6. 06 and 6. 05 μmol·L-1,respectively.
采用硅胶柱色谱、Sephadex LH - 20凝胶柱色谱、MCI柱色谱、ODS柱色谱及半制备高效液相色谱等多种色谱技术，从乌桕茎叶的95%乙醇提取物中分离得到17个化合物。通过光谱数据鉴定其结构分别为乌桕素F（1）、南五味子木脂素C（2）、刺五加酮（3）、短叶苏木酚酸乙酯（4）、7 - 羟基 - 8 - 甲氧基香豆素（5）、秦皮素（6）、秦皮苷（7）、异秦皮苷（8）、6,7,8 - 三甲氧基香豆素（9）、5,6,7,8 - 四甲氧基香豆素（10）、8 - 羟基 - 5,6,7 - 三甲氧基香豆素（11）、3,3'-二 - O - 甲基鞣花酸（12）、3,3',4'-三 - O - 甲基鞣花酸（13）、3'-甲氧基鞣花酸4'-O - α - 鼠李糖苷（14）、4,5 - 二脱氢 - 诃子酸三乙酯（15）、对映 - 贝壳杉烷 - 3 - 氧代 - 16α,17 - 二醇（16）和脱落酸（17）。化合物1为新化合物。除化合物4、11和13外，其余化合物均为首次从该植物中分离得到。对所有分离得到的化合物进行了体外抗神经炎症活性评价，结果表明化合物6和15能显著抑制脂多糖诱导的BV - 2小胶质细胞中一氧化氮的产生，IC50值分别为6.06和6.05 μmol·L-1。
