Department of Chemistry, School of Physics and Chemistry , Gwangju Institute of Science and Technology (GIST) , 123 Cheomdangwagi-ro , Buk-gu, Gwangju , 61005 , Republic of Korea.
Anal Chem. 2019 Nov 5;91(21):13772-13779. doi: 10.1021/acs.analchem.9b03210. Epub 2019 Oct 24.
Most prevalent infectious diseases worldwide are caused by mediators such as insects and characterized by high mortality and morbidity, thereby creating a global public health concern. Therefore, a sensitive, selective detection platform for diagnosing diseases in the early stages of infection is needed to prevent disease spread and to protect public health. Here, we developed novel DNA aptamers specific to the nucleocapsid protein (NP) of the severe fever with thrombocytopenia syndrome (SFTS) virus and synthesized ssDNA-binding protein-conjugated liposomes encapsulated with horseradish peroxidase (HRP) for application in a simple and universal platform. This platform achieved highly sensitive detection of the NP by measuring the colorimetric signal following lysis of the HRP encapsulated liposomes, mediated by a mixture of 3,3',5,5'-tetramethylbenzidine and HO solution. The limit of detection was 0.009 ng·mL, and NP was successfully detected in diluted human serum with a high recovery rate. Moreover, this method was specific and did not exhibit cross-reactivity among NPs of other virus types. These results demonstrated the efficacy of the proposed method as a highly sensitive, specific, and universal diagnostic tool for potential application in monitoring of the early stages of infectious diseases.
目前，全球范围内流行的传染病大多数是由昆虫等媒介传播的，具有高死亡率和高发病率的特点，这引起了全球公共卫生的关注。因此，需要一种敏感、选择性的检测平台，用于诊断感染早期的疾病，以防止疾病传播和保护公众健康。在这里，我们开发了针对严重发热伴血小板减少综合征病毒核衣壳蛋白 (NP) 的新型 DNA 适体，并合成了 ssDNA 结合蛋白偶联的包封辣根过氧化物酶 (HRP) 的脂质体，用于应用于简单而通用的平台。该平台通过测量 HRP 包封脂质体裂解后产生的比色信号，实现了对 NP 的高灵敏度检测，该信号由 3,3',5,5'-四甲基联苯胺和 HO 溶液的混合物介导。检测限为 0.009 ng·mL，并且能够以高回收率检测稀释的人血清中的 NP。此外，该方法具有特异性，不会与其他病毒类型的 NP 发生交叉反应。这些结果表明，该方法作为一种高灵敏度、特异性和通用的诊断工具，可用于监测传染病的早期阶段，具有潜在的应用前景。
