Department of Traumatology, North Medical District of Linyi People's Hospital , Linyi , PR China.
Department of Spinal Surgery, North Medical District of Linyi People's Hospital , Linyi , PR China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):4030-4037. doi: 10.1080/21691401.2019.1673766.
Spinal cord injury (SCI) can lead to varying degrees of sensory and motor dysfunction. Salvianolic acid B (Sal-B) is the dominating bioactive constituent of Danshen, which has been reported to alleviate liver fibrosis and exert neuroprotective effects. But, the influence of Sal-B in SCI remains mysterious. The research planned to delve the protective function of Sal-B in hydrogen peroxide (HO)-caused PC-12 cell injury. HO-caused PC-12 cells injury model was built, CCK-8, Transwell and flow cytometry experiments were enforced to assess cell proliferation, migration and apoptosis. The microRNA (miR)-26a plasmid and the matching control were transfected into PC-12 cells, subsequently, the influence of miR-26a inhibition in HO-corrupted PC-12 cells was evaluated. The cell growth-correlated factors and PI3K/AKT and MEK/ERK pathways were assayed through western blot assay. Results corroborated that Sal-B eased HO-evoked injury in PC-12 cells. Ascended miR-26a was monitored in Sal-B and HO-exposed cells. MiR-26a inhibition annulled the protective action of Sal-B in HO-corrupted cells. The protective function of Sal-B was enabled through activating PI3K/AKT and MEK/ERK pathways. These findings delineated that Sal-B protected PC-12 cells against HO-caused injury through ascending miR-26a initiating PI3K/AKT and MEK/ERK pathways. Highlights HO causes PC-12 cell injury; Sal-B eases HO-caused PC-12 cell injury; Sal-B protects PC-12 cells against HO-caused injury elevating miR-26a; Sal-B activates AKT and MEK/ERK pathways modulating miR-26a.
脊髓损伤 (SCI) 可导致不同程度的感觉和运动功能障碍。丹酚酸 B (Sal-B) 是丹参的主要生物活性成分，已被报道可减轻肝纤维化并发挥神经保护作用。但是，Sal-B 在 SCI 中的影响仍然是个谜。本研究旨在深入研究 Sal-B 在过氧化氢 (HO) 引起的 PC-12 细胞损伤中的保护作用。建立 HO 引起的 PC-12 细胞损伤模型，通过 CCK-8、Transwell 和流式细胞术实验评估细胞增殖、迁移和凋亡。将 miR-26a 质粒和匹配的对照转染到 PC-12 细胞中，随后评估 miR-26a 抑制对 HO 损伤的 PC-12 细胞的影响。通过 Western blot 分析测定与细胞生长相关的因子以及 PI3K/AKT 和 MEK/ERK 通路。结果证实 Sal-B 减轻了 HO 引起的 PC-12 细胞损伤。在 Sal-B 和 HO 暴露的细胞中检测到 miR-26a 上调。miR-26a 抑制消除了 Sal-B 在 HO 损伤细胞中的保护作用。Sal-B 通过激活 PI3K/AKT 和 MEK/ERK 通路发挥保护作用。这些发现表明，Sal-B 通过上调 miR-26a 激活 PI3K/AKT 和 MEK/ERK 通路来保护 PC-12 细胞免受 HO 引起的损伤。
重点 HO 导致 PC-12 细胞损伤；Sal-B 减轻 HO 引起的 PC-12 细胞损伤；Sal-B 通过上调 miR-26a 保护 PC-12 细胞免受 HO 引起的损伤；Sal-B 激活 AKT 和 MEK/ERK 通路，调节 miR-26a。
