Protocatechuic aldehyde mitigates hydrogen peroxide-triggered PC12 cell damage by down-regulating MEG3.

Protocatechuic aldehyde (PA) extracts from , which anti-oxidative and anti-inflammatory functions have been certified in diverse diseases. Nonetheless, the influence of PA in spinal cord injury (SCI) is still hazy. The research probed the function of PA in hydrogen peroxide (HO)-damaged PC12 cells. The disparate dosages of HO (0-400 µM) or PA (0-2 µM) were applied for stimulating PC12 cells, and subsequently cell viability, apoptosis, apoptosis- and autophagy-correlative factors were evaluated. After pc-MEG3 transfection, functions of MEG3 overexpression in HO and/or PA-managed PC12 cells were reassessed. Western blot was conducted to determine Wnt/β-catenin and PTEN/PI3K/AKT pathways. HO stimulation clearly triggered PC12 cell damage prohibiting cell viability and accelerating apoptosis and autophagy. But, PA management mitigated HO-triggered PC12 cells damage. Down-regulated MEG3 triggered by PA was presented in HO-managed cells. What's more, overexpressed MEG3 dramatically overturned the influences of PA in HO-damaged PC12 cells. Beyond that, PA activated Wnt/β-catenin and PTEN/PI3K/AKT repression of MEG3 in HO-managed PC12 cells. The results disclosed the protective impacts of PA on PC12 cells to resist HO-provoked damage. MEG3, Wnt/β-catenin and PTEN/PI3K/AKT pathways joined in adjusting the activity of PA in HO-damaged PC12 cells.