[The inhibitory effects of anti class II monoclonal antibodies against the proliferative response of autoreactive T-cell clone].

The role of human class II antigens in autologous mixed lymphocyte reaction (AMLR) was investigated. The AMLR between PBL as a responder and autologous EBV-transformed cells as a stimulator was inhibited by anti class II framework (7B6) and anti DR (DI-4) monoclonal antibodies. Among monoclonal antibodies specific to DQ antigens, only HKD12 showed an inhibitory effect on AMLR. Of the autoreactive T-cell clones generated by AMLR, I-3 was peculiar; its proliferative response to autologous cells was inhibited only by HKD12. The inhibitory effect of HKD12 against AMLR or I-3 clone appeared to be epitope-specific. First, the result of sequential coprecipitation assay revealed that HKD12 reacted only a part of DQ antigen. Second, HKD12 did not inhibit allogeneic MLR. Third, the proliferative response of the I-3 clone was not inhibited by 7B6 which exerted a strong inhibitory effect on the proliferative response of autoreactive T-cell clones other than I-3 as well as allogeneic MLR.