Yoshizawa K, Yano A
J Immunol. 1984 Jun;132(6):2820-9.
It has been reported that human T cells recognize the polymorphism of murine Ia antigens in the human anti-mouse xenogeneic mixed lymphocyte reactions (MLR). In this study, murine T cell recognition of human Class II antigens of the major histocompatibility complex (MHC) was analyzed in mouse anti-human xenogeneic MLR responses. The xenoreactive murine T cell proliferative response was blocked by adding anti-HLA-DR monoclonal antibody to the xenogeneic MLR culture. The specificity of xenoreactive murine T cells was examined with regard to the secondary and tertiary xenogeneic MLR system. The xenoreactive murine T cells were restimulated by distinct human stimulator cells that had no shared HLA antigens with the stimulator used in the primary MLR. The data presented here show that the murine xenoreactive T cells recognize the shared determinant(s) of HLA-DR antigen on non-T, non-B stimulator cells. The xenoreactive murine T cell proliferative responses were mediated by Thy-1+, Lyt-1+, and Lyt-2- cells. Furthermore, the xenoreactive T cell responses required Ia+ cells, and Ia antigen on accessory cells plays a crucial role in eliciting the xenoreactive responses against human stimulator cells, while Ia+ accessory cells in the responding cell population are not essential for the elicitation of allogeneic MLR responses, as reported previously.
据报道，在人类抗小鼠异种混合淋巴细胞反应（MLR）中，人类T细胞可识别小鼠Ia抗原的多态性。在本研究中，在小鼠抗人类异种MLR反应中分析了小鼠T细胞对人类主要组织相容性复合体（MHC）II类抗原的识别。通过向异种MLR培养物中添加抗HLA-DR单克隆抗体，阻断了异种反应性小鼠T细胞的增殖反应。在二级和三级异种MLR系统中检测了异种反应性小鼠T细胞的特异性。用与初次MLR中使用的刺激细胞没有共同HLA抗原的不同人类刺激细胞重新刺激异种反应性小鼠T细胞。此处给出的数据表明，小鼠异种反应性T细胞识别非T、非B刺激细胞上HLA-DR抗原的共同决定簇。异种反应性小鼠T细胞的增殖反应由Thy-1+、Lyt-1+和Lyt-2-细胞介导。此外，异种反应性T细胞反应需要Ia+细胞，辅助细胞上的Ia抗原在引发针对人类刺激细胞的异种反应中起关键作用，而如先前报道，反应细胞群体中的Ia+辅助细胞对于同种异体MLR反应的引发并非必不可少。
