School of Water Conservancy Engineering, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, Henan, China.
Zhengzhou Key Laboratory of Water Resource and Environment, Zhengzhou, 450001, China.
Bull Environ Contam Toxicol. 2019 Dec;103(6):814-821. doi: 10.1007/s00128-019-02728-5. Epub 2019 Oct 12.
To understand the toxic mechanism of ammonia and identify effective biomarkers on the oxidative stress for the fish Carassius auratus red var., acute and chronic toxicity tests were conducted. The 96-h LC of total ammonia nitrogen (TAN) for C. auratus was 135.4 mg L, the corresponding unionized ammonia (NH) concentration was 1.5 mg L. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione-peroxidase (GSH-Px) and glutathione (GSH) showed an increase with a subsequent falling, while the malondialdehyde (MDA) increased during the chronic test. The SOD, MDA, and GSH could be effective biomarkers to evaluate the TAN oxidative stress, the maximum acceptable toxicant concentration (MATC) was 11.3 mg L for TAN. To our knowledge, this is the first study to propose biomarkers to evaluate potential environmental risk and establish a risk threshold for TAN in C. auratus.
为了了解氨的毒性机制，并确定鲤鱼（Carassius auratus red var.）氧化应激的有效生物标志物，进行了急性和慢性毒性试验。鲤鱼的总氨氮（TAN）96 小时半数致死浓度为 135.4mg/L，相应的非离子氨（NH）浓度为 1.5mg/L。超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽（GSH）的活性先升高后降低，而丙二醛（MDA）在慢性试验中增加。SOD、MDA 和 GSH 可作为评估 TAN 氧化应激的有效生物标志物，TAN 的最大可接受毒物浓度（MATC）为 11.3mg/L。据我们所知，这是首次提出生物标志物来评估 TAN 在鲤鱼中的潜在环境风险并建立风险阈值的研究。
