中华眼镜蛇毒十五肽对环磷酰胺诱导的肝损伤的保护作用。

Protective effects of pentadecapeptide derived from Cyclaina sinensis against cyclophosphamide-induced hepatotoxicity.

机构信息

Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China.

Hangzhou Obstetrics & Gynecology Hospital, Hangzhou, 310008, China.

出版信息

Biochem Biophys Res Commun. 2019 Dec 3;520(2):392-398. doi: 10.1016/j.bbrc.2019.10.051. Epub 2019 Oct 10.

DOI:10.1016/j.bbrc.2019.10.051

PMID:31607481

Abstract

Our study was aimed at investigating the hepatoprotective effects of pentadecapeptide (RVAPEEHPVEGRYLV) from Cyclaina sinensis (SCSP) against cyclophosphamide (CTX)-induced hepatotoxicity in mice. Our results show that SCSP can significantly alleviate CTX-induced hepatotoxicity by decreasing the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG) and malondialdehyde (MDA), and increasing the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) in the liver. In addition, the levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) were also significantly decreased in the liver tissues when treated with SCSP. Moreover, the protein levels of the toll-like receptor 4 (TLR4)-mediated nuclear factor-kappa B (NF-κB) pathway and apoptosis-related proteins were also restored by SCSP treatment. Overall, our results suggest that SCSP can potentially improve the CTX-induced hepatotoxicity.

摘要

本研究旨在探讨中华匙吻鲟环五肽（RVAPEEHPVEGRYLV）对环磷酰胺（CTX）诱导的小鼠肝损伤的保护作用。结果表明，SCSP 可通过降低丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、甘油三酯（TG）和丙二醛（MDA）水平，增加超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）水平，显著减轻 CTX 诱导的肝损伤。此外，SCSP 还可降低肝组织中白细胞介素（IL）-1β、IL-6 和肿瘤坏死因子-α（TNF-α）的水平。SCSP 还可通过恢复 Toll 样受体 4（TLR4）介导的核因子-κB（NF-κB）通路和凋亡相关蛋白的水平，改善 CTX 诱导的肝损伤。总之，SCSP 可能对 CTX 诱导的肝损伤具有潜在的改善作用。