Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Health, Beijing Technology and Business University, Beijing 100048, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China.
Nutrients. 2022 Oct 25;14(21):4491. doi: 10.3390/nu14214491.
The carnosine and anserine, which represent histidine dipeptides (HD), are abundant in chicken broth (CB). HD are endogenous dipeptide that has excellent antioxidant and immunomodulatory effects. The immunomodulatory effect of CB hydrolysate (CBH) and HD in cyclophosphamide (CTX)-induced immunosuppressed mice was examined in this study. CBH and HD were given to mice via oral gavage for 15 days, accompanied by intraperitoneal CTX administration to induce immunosuppression. CBH and HD treatment were observed to reduce immune organ atrophy (p < 0.05) and stimulate the proliferation of splenic lymphocytes (p < 0.05) while improving white blood cell, immunoglobulin M (IgM), IgG, and IgA levels (p < 0.05). Moreover, CBH and HD strongly stimulated interleukin-2 (IL-2) and interferon-gamma (IFN-γ) production by up-regulating IL-2 and IFN-γ mRNA expression (p < 0.05) while inhibiting interleukin-10 (IL-10) overproduction and IL-10 mRNA expression (p < 0.05). In addition, CBH and HD prevented the inhibition of the nitric oxide (NP)/cyclic guanosine monophosphate-cyclic adenosine monophosphate (cGMP-cAMP)/protein kinase A (PKA) signaling pathway (p < 0.05). These results indicate that CBH and HD have the potential to prevent immunosuppression induced by CTX. Our data demonstrate that CBH can effectively improve the immune capacity of immunosuppressed mice similar to the same amount of purified HD, which indicates that CBH plays its role through its own HD.
肌肽和鹅肌肽是组氨酸二肽（HD）的代表，在鸡汤（CB）中含量丰富。HD 是一种内源性二肽，具有出色的抗氧化和免疫调节作用。本研究旨在研究 CB 水解物（CBH）和 HD 对环磷酰胺（CTX）诱导的免疫抑制小鼠的免疫调节作用。通过灌胃给予小鼠 CBH 和 HD15 天，同时腹腔注射 CTX 以诱导免疫抑制。结果表明，CBH 和 HD 处理可减少免疫器官萎缩（p < 0.05）并刺激脾淋巴细胞增殖（p < 0.05），同时提高白细胞、免疫球蛋白 M（IgM）、IgG 和 IgA 水平（p < 0.05）。此外，CBH 和 HD 通过上调 IL-2 和 IFN-γ mRNA 表达强烈刺激白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）的产生（p < 0.05），同时抑制白细胞介素-10（IL-10）的过度产生和 IL-10 mRNA 表达（p < 0.05）。此外，CBH 和 HD 可防止抑制一氧化氮（NO）/环鸟苷酸单磷酸-环磷酸腺苷（cGMP-cAMP）/蛋白激酶 A（PKA）信号通路（p < 0.05）。这些结果表明，CBH 和 HD 具有预防 CTX 诱导的免疫抑制的潜力。我们的数据表明，CBH 可以有效提高免疫抑制小鼠的免疫能力，与相同量的纯化 HD 相似，这表明 CBH 通过自身的 HD 发挥作用。
