Al Kindi Arwa, Alkahtani Abdullah M, Nalubega Mayimuna, El-Chami Cecile, O'Neill Catherine, Arkwright Peter D, Pennock Joanne L
Lydia Becker Institute of Immunology and Inflammation, The University of Manchester, Manchester, United Kingdom.
Division of Infection, Immunity and Respiratory Medicine, The University of Manchester, Manchester, United Kingdom.
Front Microbiol. 2019 Sep 24;10:2242. doi: 10.3389/fmicb.2019.02242. eCollection 2019.
causes the majority of skin and soft tissue infections. Half of patients treated for primary skin infections suffer recurrences within 6 months despite appropriate antibiotic sensitivities and infection control measures. We investigated whether internalized by human skin keratinocytes are effectively eradicated by standard anti-staphylococcal antibiotics. , but not , were internalized and survive within keratinocytes without inducing cytotoxicity or releasing the IL-33 danger signal. Except for rifampicin, anti-staphylococcal antibiotics in regular clinical use, including flucloxacillin, teicoplanin, clindamycin, and linezolid, did not kill internalized , even at 20-fold their standard minimal inhibitory concentration. We conclude that internalization of by human skin keratinocytes allows the bacteria to evade killing by most anti-staphylococcal antibiotics. Antimicrobial strategies, including antibiotic combinations better able to penetrate into mammalian cells are required if intracellular are to be effectively eradicated and recurrent infections prevented.
引起大多数皮肤和软组织感染。尽管有适当的抗生素敏感性和感染控制措施，但接受原发性皮肤感染治疗的患者中有一半在6个月内复发。我们研究了被人皮肤角质形成细胞内化的[细菌名称未给出]是否能被标准的抗葡萄球菌抗生素有效根除。[细菌名称未给出]能被内化并在角质形成细胞内存活，且不诱导细胞毒性或释放IL - 33危险信号，但[另一种细菌名称未给出]则不能。除利福平外，常规临床使用的抗葡萄球菌抗生素，包括氟氯西林、替考拉宁、克林霉素和利奈唑胺，即使在其标准最小抑菌浓度的20倍时也不能杀死内化的[细菌名称未给出]。我们得出结论，人皮肤角质形成细胞对[细菌名称未给出]的内化使细菌能够逃避大多数抗葡萄球菌抗生素的杀灭作用。如果要有效根除细胞内的[细菌名称未给出]并预防复发性感染，就需要包括能更好地穿透哺乳动物细胞的抗生素组合在内的抗菌策略。
