Matera L, Francis M K, Herlyn M, Trucco M, Santoli D
Nat Immun Cell Growth Regul. 1985;4(2):90-102.
A number of human lymphoid and non-lymphoid leukemic cell lines differing for expression of HLA-DR antigens were analyzed for the ability to induce natural killer(NK)-like activity and proliferation in lymphocytes from healthy donors. The ability to elicit the generation of NK-like activity in the responder lymphocytes varied greatly depending upon the type of antimitotic treatment (gamma-irradiation versus mitomycin C) received by the tumor cells prior to the start of the mixed cultures. By contrast, the induction of T-cell proliferation was positively correlated with the presence of DR molecules on the tumor cell lines. Nevertheless, DR- leukemic cells pretreated with the appropriate antimitotic agent did induce a proliferative response in the mixed cultures. T lymphocytes cultured without stimulator cells in spent medium containing high levels of cell-free DR antigens failed to undergo blastogenesis and proliferation, indicating that DR antigens can function as stimulatory molecules only when they are cell-associated.
对若干表达HLA - DR抗原情况不同的人淋巴细胞和非淋巴细胞白血病细胞系进行了分析，以研究它们诱导健康供者淋巴细胞产生自然杀伤（NK）样活性和增殖的能力。在混合培养开始前，肿瘤细胞接受的抗有丝分裂处理类型（γ射线照射与丝裂霉素C）不同，引发应答淋巴细胞产生NK样活性的能力差异很大。相比之下，T细胞增殖的诱导与肿瘤细胞系上DR分子的存在呈正相关。然而，用适当抗有丝分裂剂预处理的DR阴性白血病细胞在混合培养中确实诱导了增殖反应。在含有高水平无细胞DR抗原的用过的培养基中，无刺激细胞培养的T淋巴细胞未能发生母细胞化和增殖，这表明DR抗原只有与细胞相关时才能作为刺激分子发挥作用。
