Isolation of lambda phage DNA by hydroxylapatite chromatography.
作者信息
Ivanov I, Gigova L
出版信息
Anal Biochem. 1985 May 1;146(2):389-92. doi: 10.1016/0003-2697(85)90556-1.
PMID:3161411
Abstract
A simple and rapid (1 day) method for preparation of lambda phage DNA was proposed. The method included two main steps: (a) growth and lysis of bacteria containing lambda phage and (b) purification of lambda phage DNA by hydroxylapatite chromatography. The phage DNA prepared by this method was intact and free of RNA, proteins, and bacterial DNA.
摘要
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