Galán J E, Timoney J F
Department of Veterinary Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Infect Immun. 1990 Sep;58(9):3116-21. doi: 10.1128/iai.58.9.3116-3121.1990.
Erysipelothrix rhusiopathiae is a primary pathogen of swine and turkeys and sporadic cause of disease in a variety of other hosts, including humans. A genomic library of the highly virulent strain of E. rhusiopathiae E1-6P was constructed in the expression-cloning vector lambda gt11 and screened with serum from a pig convalescent from an E. rhusiopathiae experimental infection. Immunoreactive clones were screened for their ability to protectively immunized mice. Two clones, lambda gt11/ersA and lambda gt11/ersB, were obtained that protected mice against challenge with E. rhusiopathiae E1-6P. Antisera against the recombinant clones reacted with polypeptides of molecular weights 66,000, 64,000, and 43,000 in detergent-solubilized surface antigen preparations and whole-cell lysates of E. rhusiopathiae. These polypeptides were also the major antigens recognized by convalescent pig serum when reacted with the same preparations. Western immunoblot and Southern blot analysis revealed that the cloned genes and gene products were present in all of the E. rhusiopathiae strains tested.
猪红斑丹毒丝菌是猪和火鸡的主要病原体，也是包括人类在内的多种其他宿主中疾病的散发病因。在表达克隆载体λgt11中构建了高毒力猪红斑丹毒丝菌菌株E1-6P的基因组文库，并用来自猪红斑丹毒丝菌实验感染康复猪的血清进行筛选。筛选出具有免疫反应性的克隆，检测其对小鼠进行保护性免疫的能力。获得了两个克隆，λgt11/ersA和λgt11/ersB，它们能保护小鼠免受猪红斑丹毒丝菌E1-6P的攻击。针对重组克隆的抗血清与猪红斑丹毒丝菌去污剂溶解表面抗原制剂和全细胞裂解物中分子量为66,000、64,000和43,000的多肽发生反应。当与相同制剂反应时，这些多肽也是康复猪血清识别的主要抗原。蛋白质免疫印迹和Southern印迹分析表明，克隆的基因和基因产物存在于所有测试的猪红斑丹毒丝菌菌株中。
