Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku 160-8582, Tokyo, Japan.
Department of Ophthalmology, Tsurumi University School of Dental Medicine, Tsurumi-ku 230-8501, Kanagawa, Japan.
Int J Mol Sci. 2019 Oct 13;20(20):5078. doi: 10.3390/ijms20205078.
The use of eyewash solutions in Japan, especially in patients with allergic conjunctivitis and contact lens wearers, has been increasing. Our aim was to investigate how the use of preservative-free eyewash solution in healthy eyes for one month affects corneal safety and ocular surface mucin. We analyzed 42 eyes of 21 individuals (17 males, four females; mean age: 36.1 ± 7.4 years) without ocular allergies, dry eyes, or other ocular diseases through a prospective study. Eyes were randomized to a wash group (group one) and a nonwash follow up group (group two). We evaluated the dry eye-related quality-of-life score (DEQS), tear film breakup time (TBUT), fluorescein staining score, mRNA expression of MUC5AC and MUC16, MUC16 immunohistochemistry, and MUC5AC periodic acid Schiff (PAS) staining. There was a significant decrease in DEQS scores after one month of eyewash use ( < 0.05). There were no significant differences in other evaluation items that were analyzed (all > 0.05). Furthermore, no significant differences were observed between group one and group two in all endpoints (all > 0.05). The results suggest that one month use of a nonpreserved eyewash solution has no detrimental effects on the tear film and the ocular surface mucins.
在日本，人们越来越多地使用洗眼液，特别是在过敏性结膜炎和隐形眼镜佩戴者中。我们的目的是研究在健康眼睛中使用不含防腐剂的洗眼液一个月会如何影响角膜安全性和眼表面黏液。我们通过一项前瞻性研究分析了 42 只眼睛（21 个人，17 名男性，4 名女性；平均年龄：36.1 ± 7.4 岁），这些眼睛均无眼部过敏、干眼症或其他眼部疾病。我们将眼睛随机分为洗眼组（一组）和非洗眼随访组（二组）。我们评估了与干眼症相关的生活质量评分（DEQS）、泪膜破裂时间（TBUT）、荧光素染色评分、MUC5AC 和 MUC16 的 mRNA 表达、MUC16 免疫组化和 MUC5AC 过碘酸雪夫（PAS）染色。使用洗眼液一个月后，DEQS 评分显著下降（ < 0.05）。分析的其他评估项目没有显著差异（均 > 0.05）。此外，所有终点在一组和二组之间均无显著差异（均 > 0.05）。结果表明，使用不含防腐剂的洗眼液一个月对泪膜和眼表面黏液没有不良影响。
