Furlan João Pedro Rueda, de Almeida Otávio Guilherme Gonçalves, De Martinis Elaine Cristina Pereira, Stehling Eliana Guedes
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
Front Microbiol. 2019 Sep 18;10:2151. doi: 10.3389/fmicb.2019.02151. eCollection 2019.
complex is considered one of the main causes of hospital-acquired infections. was recently characterized within this complex and it has been described as an emergent pathogen associated with bacteremia. The emergence of multidrug-resistant (MDR) bacteria, including sp., is considered a global public health threat and an environmental problem because MDR bacteria have been spreading from several sources. Therefore, this study aimed to characterize an environmental MDR isolate (SAb133) using whole genome sequencing and a comparative genomic analysis was performed with strains recovered from various sources. The SAb133 isolate was obtained from soil of a corn crop field and presented high MICs for antimicrobials and metals. The comparative genomic analyses revealed ANI values higher than 95% of relatedness with other strains than complex. Resistome and virulome analyses were also performed and showed different antimicrobial resistance determinants and metal tolerance genes as well as virulence genes related to known virulence genes. In addition, genomic islands, IS elements, plasmids and prophage-related sequences were detected. Comparative genomic analysis showed that MDR SAb133 had a high amount of determinants related to antimicrobial resistance and tolerance to metals, besides the presence of virulence genes. To the best of our knowledge, this is the first report of a whole genome sequence of a MDR isolated from soil. Therefore, this study contributed to a better understanding of the genetic relationship among the few known strains worldwide distributed.
复合体被认为是医院获得性感染的主要原因之一。最近在该复合体内对其进行了表征，并且它已被描述为与菌血症相关的新兴病原体。包括[具体菌种]在内的多重耐药（MDR）细菌的出现被视为全球公共卫生威胁和环境问题，因为MDR细菌已从多个来源传播。因此，本研究旨在使用全基因组测序对一株环境MDR[具体菌种]分离株（SAb133）进行表征，并对从各种来源回收的[具体菌种]菌株进行比较基因组分析。SAb133分离株从玉米作物田的土壤中获得，对抗菌药物和金属表现出高最低抑菌浓度（MIC）。比较基因组分析显示，与其他[具体菌种]菌株相比，与[具体复合体]的ANI值高于95%的相关性。还进行了耐药基因组和毒力基因组分析，结果显示了不同的抗菌耐药决定因素和金属耐受基因以及与已知毒力基因相关的毒力基因。此外，还检测到基因组岛、插入序列（IS）元件、质粒和原噬菌体相关序列。比较基因组分析表明，MDR[具体菌种]SAb133除了存在毒力基因外，还具有大量与抗菌耐药性和金属耐受性相关的决定因素。据我们所知，这是首次报道从土壤中分离出的MDR[具体菌种]的全基因组序列。因此，本研究有助于更好地理解全球分布的少数已知[具体菌种]菌株之间的遗传关系。
