Department of Pulmonary and Critical Care Medicine, Peking University Third Hospital, Beijing 100191, China.
Tianjin Key Lab. of Metabolic Diseases and Department of Physiology, Tianjin Medical University, Tianjin 300070, China.
Biomed Environ Sci. 2019 Sep;32(9):659-672. doi: 10.3967/bes2019.085.
Asthma and chronic obstructive pulmonary disease (COPD) feature different inflammatory and cellular profiles in the airways, indicating that the cellular metabolic pathways regulating these disorders are distinct.
We aimed to compare the serum metabolomic profiles among mild persistent asthmatic patients, individuals with stable COPD, and healthy subjects and to explore the potential metabolic biomarkers and pathways. The serum metabolomic profiles of 17 subjects with mild persistent asthma, 17 subjects with stable COPD, and 15 healthy subjects were determined by an untargeted metabolomic analysis utilizing liquid chromatography-mass spectrometry. A series of multivariate statistical analyses was subsequently used.
Multivariate analysis indicated a distinct separation between the asthmatic patients and healthy controls in electrospray positive and negative ions modes, respectively. A total of 19 differential metabolites were identified. Similarly, a distinct separation between asthma and COPD subjects was detected in the two ions modes. A total of 16 differential metabolites were identified. Among the identified metabolites, the serum levels of hypoxanthine were markedly higher in asthmatic subjects compared with those in COPD or healthy subjects.
Patients with asthma present a unique serum metabolome, which can distinguish them from individuals with COPD and healthy subjects. Purine metabolism alteration may be distinct and involved in the pathogenesis of asthma.
哮喘和慢性阻塞性肺疾病（COPD）在气道中具有不同的炎症和细胞特征，表明调节这些疾病的细胞代谢途径是不同的。
我们旨在比较轻度持续性哮喘患者、稳定期 COPD 患者和健康受试者的血清代谢组学特征，并探讨潜在的代谢生物标志物和途径。通过利用液相色谱-质谱联用的非靶向代谢组学分析，确定了 17 名轻度持续性哮喘患者、17 名稳定期 COPD 患者和 15 名健康受试者的血清代谢组学特征。随后使用一系列多变量统计分析。
多变量分析表明，在电喷雾正离子和负离子模式下，哮喘患者与健康对照组分别有明显的分离。共鉴定出 19 种差异代谢物。同样，在两种离子模式下，哮喘和 COPD 患者之间也存在明显的分离。共鉴定出 16 种差异代谢物。在鉴定出的代谢物中，与 COPD 或健康受试者相比，哮喘患者的血清次黄嘌呤水平明显升高。
哮喘患者具有独特的血清代谢组学特征，可将其与 COPD 患者和健康受试者区分开来。嘌呤代谢的改变可能是独特的，并参与哮喘的发病机制。
