Chang Chun, Guo Zhi-guo, He Bei, Yao Wan-zhen
Department of Respiratory Medicine, Peking University Third Hospital, Beijing 100191, China.
Department of Emergency Medicine, Peking University Third Hospital, Beijing 100191, China.
Acta Pharmacol Sin. 2015 Nov;36(11):1356-66. doi: 10.1038/aps.2015.102.
To character the specific metabolomics profiles in the sera of Chinese patients with mild persistent asthma and to explore potential metabolic biomarkers.
Seventeen Chinese patients with mild persistent asthma and age- and sex-matched healthy controls were enrolled. Serum samples were collected, and serum metabolites were analyzed using GC-MS coupled with a series of multivariate statistical analyses.
Clear intergroup separations existed between the asthmatic patients and control subjects. A list of differential metabolites and several top altered metabolic pathways were identified. The levels of succinate (an intermediate in tricarboxylic acid cycle) and inosine were highly upregulated in the asthmatic patients, suggesting a greater effort to breathe during exacerbation and hypoxic stress due to asthma. Other differential metabolites, such as 3,4-dihydroxybenzoic acid and phenylalanine, were also identified. Furthermore, the differential metabolites possessed higher values of area under the ROC curve (AUC), suggesting an excellent clinical ability for the prediction of asthma.
Metabolic activity is significantly altered in the sera of Chinese patients with mild persistent asthma. The data might be helpful for identifying novel biomarkers and therapeutic targets for asthma.
描绘中国轻度持续性哮喘患者血清中的特定代谢组学特征，并探索潜在的代谢生物标志物。
招募了17名中国轻度持续性哮喘患者以及年龄和性别匹配的健康对照者。采集血清样本，使用气相色谱-质谱联用技术结合一系列多元统计分析方法对血清代谢物进行分析。
哮喘患者与对照受试者之间存在明显的组间分离。确定了一系列差异代谢物和几个变化最显著的代谢途径。琥珀酸（三羧酸循环中的一种中间体）和肌苷的水平在哮喘患者中高度上调，这表明哮喘发作时呼吸更加费力以及存在低氧应激。还鉴定出了其他差异代谢物，如3,4-二羟基苯甲酸和苯丙氨酸。此外，差异代谢物的ROC曲线下面积（AUC）值更高，表明其对哮喘具有出色的临床预测能力。
中国轻度持续性哮喘患者血清中的代谢活性发生了显著改变。这些数据可能有助于识别哮喘的新型生物标志物和治疗靶点。
