Medical Genetics Laboratory, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia.
Perdana University School of Foundation Studies, Perdana University, Selangor Darul Ehsan, Malaysia.
Dis Markers. 2019 Sep 15;2019:3875147. doi: 10.1155/2019/3875147. eCollection 2019.
The ceramide synthase 2 () gene has been linked to tumour recurrence and invasion in many different types of cancers including bladder cancer. In this study, the expression levels of in bladder cancer cell lines were analysed using qRT-PCR and the protein expression in clinical bladder cancer histopathological specimens were examined via immunohistochemistry. The potential utility of as a predictive biomarker of response to oncolytic virotherapy was assessed by correlating the mRNA expression to IC values of cells treated with the Newcastle disease virus (NDV), AF2240 strain. This study demonstrates that is differentially expressed in different types of bladder cancer cell lines and that the siRNA-mediated downregulation of the expression of reduces the migratory potential of UMUC1 bladder cancer cells. However, there were no significant correlations between the expression levels of the CERS2 protein with bladder cancer grade/stage or between the IC50 values of cells treated with NDV and expression. Although the utility of expression may be limited, its potential as an antimigration cancer therapeutic should be further examined.
神经酰胺合酶 2 () 基因已与多种癌症（包括膀胱癌）的肿瘤复发和侵袭有关。在这项研究中，使用 qRT-PCR 分析膀胱癌细胞系中的表达水平，并通过免疫组织化学检查临床膀胱癌组织病理学标本中的蛋白表达。通过将 mRNA 表达与用新城疫病毒 (NDV)、AF2240 株处理的细胞的 IC 值相关联，评估了 作为溶瘤病毒治疗反应的预测生物标志物的潜在效用。本研究表明，在不同类型的膀胱癌细胞系中，表达水平不同，siRNA 介导的 表达下调降低了 UMUC1 膀胱癌细胞的迁移潜力。然而，CERS2 蛋白的表达水平与膀胱癌分级/分期之间或用 NDV 处理的细胞的 IC50 值与 表达之间没有显著相关性。尽管 的表达可能具有一定的局限性，但作为一种抗迁移癌症治疗的潜力应该进一步研究。
